Protein Info for CA264_07150 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 2 to 180 (179 residues), 184.6 bits, E=3e-58
• TIGR01393: elongation factor 4
  • amino acids 2 to 594 (593 residues), 994 bits, E=2.2e-303
• TIGR00231: small GTP-binding protein domain
  • amino acids 5 to 173 (169 residues), 63.5 bits, E=2e-21
• PF03144: GTP_EFTU_D2
  • amino acids 207 to 274 (68 residues), 44.6 bits, E=3.1e-15
• PF00679: EFG_C
  • amino acids 397 to 483 (87 residues), 63.7 bits, E=2.7e-21
• PF06421: LepA_C
  • amino acids 486 to 592 (107 residues), 177 bits, E=1.9e-56

Best Hits

Swiss-Prot: 84% identical to LEPA_CYTH3: Elongation factor 4 (lepA) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 84% identity to chu:CHU_1717)

MetaCyc: 57% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A1X9YQT4 at UniProt or InterPro

Protein Sequence (594 amino acids)

>CA264_07150 elongation factor 4 (Pontibacter actiniarum KMM 6156, DSM 19842)
MKNIRNFCIIAHIDHGKSTLADRLLEFTQTVAEREMQHQLLDNMDLERERGITIKSHAIQ
MNYHYKGEDYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVDSSQGIEAQTISNLYLAIG
NDLEIIPVLNKIDLPHAMPEEVSDQIIDLIGCDKEDIIHASGKAGIGVEDILNAICDRIP
APKGDPEAPLQALIFDSVFNSYRGIEVYFRIFNGSLKKGEKVKFINTGKTYEADEIGVLK
LNQEARQEMGAGNVGYLISGIKNAKEVKVGDTITHVDRPAQGIQGFEDVKPMVFAGIYPV
ETSEYEELRASMEKLQLNDASLVWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMT
VITTVPSVQFHAYTTKEAMVKVNAPSEMPDPNYIDHIEEPFIKAQIISKSDFVGPIITLC
MDKRGILKNQTYLTSDRVEMTFEMPLAEIVFDFFDKLKTISRGYASLDYELIGFRQSNMV
KLDIMLNGEKVDALSAIVHRDKAYDWGKRLCEKLRELLPRQMFEIAIQAAIGQKIIARET
VKALRKNVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFLAVLKLD