Protein Info for CA264_05585 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: pyridoxamine 5'-phosphate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 215 TIGR00558: pyridoxamine 5'-phosphate oxidase" amino acids 6 to 215 (210 residues), 310.4 bits, see alignment E=3.1e-97 PF12766: Pyridox_oxase_2" amino acids 32 to 115 (84 residues), 30.4 bits, see alignment E=7.4e-11 PF01243: Putative_PNPOx" amino acids 36 to 119 (84 residues), 89.7 bits, see alignment E=1.7e-29 PF10590: PNP_phzG_C" amino acids 175 to 215 (41 residues), 73.6 bits, see alignment 1.4e-24

Best Hits

Swiss-Prot: 58% identical to PDXH_BURM1: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 59% identity to bgd:bgla_1g32760)

MetaCyc: 47% identical to pyridoxine/pyridoxamine 5'-phosphate oxidase (Escherichia coli K-12 substr. MG1655)
Pyridoxal 5'-phosphate synthase. [EC: 1.4.3.5]; 1.4.3.5 [EC: 1.4.3.5]

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.5

Use Curated BLAST to search for 1.4.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YQ37 at UniProt or InterPro

Protein Sequence (215 amino acids)

>CA264_05585 pyridoxamine 5'-phosphate oxidase (Pontibacter actiniarum KMM 6156, DSM 19842)
MALTHNIADIRINYTKQALTEASVLQDPVQQFQVWLQEALESEVHEPTALVLSTVGGGKP
SARVVLLKGVDEQGFIFFTNYESRKGHDLAENPYASLTFFWPELERQVRVEGKVEKVAPA
LSDSYFHSRPKGSQIGAWASPQSRVIEAREVLEQREQQYSSQFAREEQVPRPAHWGGYRL
LPHYLEFWQGRPSRLHDRIVYEREGGNWQVKRLAP