Protein Info for CA264_05540 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 TIGR01063: DNA gyrase, A subunit" amino acids 7 to 811 (805 residues), 1170.4 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 31 to 470 (440 residues), 588.7 bits, see alignment E=7.3e-181 PF03989: DNA_gyraseA_C" amino acids 503 to 549 (47 residues), 38.3 bits, see alignment 7.2e-14 amino acids 554 to 602 (49 residues), 34.6 bits, see alignment 1.1e-12 amino acids 615 to 659 (45 residues), 28.2 bits, see alignment 1e-10 amino acids 669 to 709 (41 residues), 36.1 bits, see alignment 3.5e-13 amino acids 715 to 758 (44 residues), 44.4 bits, see alignment 9.1e-16 amino acids 765 to 811 (47 residues), 45.9 bits, see alignment 3.2e-16

Best Hits

Swiss-Prot: 56% identical to GYRA_BACHD: DNA gyrase subunit A (gyrA) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 74% identity to mtt:Ftrac_0499)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YQ09 at UniProt or InterPro

Protein Sequence (854 amino acids)

>CA264_05540 DNA gyrase subunit A (Pontibacter actiniarum KMM 6156, DSM 19842)
MTEGERIIPINIEDEMRGAYIDYSMSVIISRALPDVRDGLKPVHRRVLYGMSELGVSYNK
SYKKSARIVGEVLGKYHPHGDSSVYETMVRMAQEWSLRYPLVDGQGNYGSIDGDSPAAMR
YTEARLKRIADELLADLDKNTVDFVPNFDDSLKEPSVMPAKLPNLLVNGTSGIAVGMATN
MAPHNLTEVVNGITAYIDNRDITIAELMQYITAPDFPTGGIIHGYDGVKSAFETGRGRVL
MRARANFETTPSGKEQIIVTEIPYMVNKATLIEKTAALINEKKIEGISDLRDESDRDGLR
IVYDLKRDAIPNVVLNNLYKYTALQSSFGVNNVALVKGRPMTLNLKELIQYFVEHRHEVV
VRRTQYELDEAKKRAHILEGLLIALDNLDEVINLIRSSRDPEVARNGLIERFQLSEIQAR
AILDMRLQRLTGLERDKIQQEYEEIMKLIDYLTSILNDEGLRMQIIKDELAEIKERYGDG
RRTGIEASTGDISYEDMIPEENMVITISHEGYIKRTSLNEYRSQSRGGVGSRGVAASKDS
DFTEHLFIANTHHHMLFFTEFGRVFWLKVYEIPEGGKTTKGRAIQNLIQIEKDDKVRAVL
NVHDLKNQDYVLNHNLVFITEQGTIKKTVLEAYSRPRTNGINAISINDGDRLLDVQLTNT
SSEIVIALESGRAIRFNEDQVRPMGRTAAGVRAVTLAGPDDKVVGMVCVENENTDLLVVS
EKGFGKRSLLEEYRITNRGGKGVKTMNVTDKTGKLVAIKGVVDTDDLMIINRSGITIRLR
VGELRVIGRATQGVRLIKLTEGDQISSVAKVEVENEEEVEELLMEQSEPSPEDTLNPDDM
IDPETTEPEEPEEA