Protein Info for CA264_05530 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: phosphoenolpyruvate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00311: PEPcase
  • amino acids 114 to 301 (188 residues), 81.6 bits, E=1.7e-27
  • amino acids 318 to 847 (530 residues), 414.3 bits, E=5e-128

Best Hits

KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 64% identity to cpi:Cpin_1471)

Predicted SEED Role

"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)

MetaCyc Pathways

• nitrogen remobilization from senescing leaves (7/8 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
• mixed acid fermentation (10/16 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.31

Sequence Analysis Tools

See A0A1X9YPZ0 at UniProt or InterPro

Protein Sequence (861 amino acids)

>CA264_05530 phosphoenolpyruvate carboxylase (Pontibacter actiniarum KMM 6156, DSM 19842)
MNRINQHTSSAVEVFRSEVGTRFEMYNSLFSSLPFHRVEKTGVLLSLFVLHCEEGYQRHL
SPRKIISSFFEQYTTYRTDQEQHDLLFRFVQYAERQVVLFDALEEAAFRDTHDMYGVGTL
KQLGAAVVQANAEPRLAEKLKNFAVRLVLTAHPTQFYPSNVLGIINDLSQALATDNTSLA
HSYLRQLGKTPFFKKEKPTPYEEAVNLIWFLENVFYKAGGNILTSLKNQFPEAVQRNSQL
VRMGFWPGGDRDGNPFVKADTTLRVADALRGAVVRSYYADVRRLKRRLTFKGVESLLKEL
ETKLYNNLFVPGYSFDLTKEDILDTLRQISALLIEEHSSLFLNQVEALINRVELFGLYFA
SLDIRQDSSVHTHLFEELSLVTDAIPGDYTEQSEEEKINLLRSVSEQVDPAVLEDETLRD
TLEVIGAIKTIQEYNGEEGCHRYIISHSTSALNVMEVYTLFRLGGWQPEELTVDIVPLFE
TIADLGHAAGVMRELYENEVYRSHLHRRHNIQTIMLGFSDGTKDGGYFMANWSIYKAKEA
LTQVSREYGLEVIFFDGRGGPPARGGGKTQQFYASMGSNIANQEIQLTVQGQTISSNFGN
VDSAQFNMEQLIHAGISNALFSQKGATLSGQEDTLLQQLANISYEAYSKLKNHRVFLEYL
NYISPLRYYGEANIGSRPSKRKAGKLNLDDLRAVPYVGAWSQLKQNLPGYYGVGIALEKM
HESGRWEFLQKLYKESLYFKTLLDNCEMAMKKCFFPVTAYLAEHPKYGELWRIIHEEYER
TRKYVLMLSQASELMADKPIEQLSIQTRQRIELPLITIQQYALANIREIEEQHPQSQAKQ
VYEKLVVRCSFGIINAERNSA