Protein Info for CA264_04970 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: tRNA (guanine(46)-N(7))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 TIGR00091: tRNA (guanine-N(7)-)-methyltransferase" amino acids 31 to 211 (181 residues), 141.5 bits, see alignment E=1e-45 PF01209: Ubie_methyltran" amino acids 40 to 153 (114 residues), 22.7 bits, see alignment E=1.4e-08 PF13847: Methyltransf_31" amino acids 41 to 158 (118 residues), 44.2 bits, see alignment E=4.5e-15 PF02390: Methyltransf_4" amino acids 42 to 209 (168 residues), 119.5 bits, see alignment E=2.7e-38 PF13649: Methyltransf_25" amino acids 45 to 120 (76 residues), 37.1 bits, see alignment E=1.1e-12

Best Hits

Swiss-Prot: 51% identical to TRMB_CYTH3: tRNA (guanine-N(7)-)-methyltransferase (trmB) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K03439, tRNA (guanine-N7-)-methyltransferase [EC: 2.1.1.33] (inferred from 51% identity to chu:CHU_1543)

Predicted SEED Role

"tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)" (EC 2.1.1.33)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YPU2 at UniProt or InterPro

Protein Sequence (217 amino acids)

>CA264_04970 tRNA (guanine(46)-N(7))-methyltransferase (Pontibacter actiniarum KMM 6156, DSM 19842)
MGRSKLAKFAAIAERENVIEPGDALYGQLRGKWRELHFENNNPVVLEVGCGRGEYTIGMA
RMFPDTNFIGSDIKGARLWKGSTMAEEEELDNVTFLRTFIEQLEENFAENELDQIWITFP
DPRPRDRDIKRRLTSPRFLDMYEKVLKPGGIVHFKTDNEALFDYTLEILAERKPKDLLYT
KDLYNSDLQEHTMGIYTTYEKRYLGEGIPIKYLQYKV