Protein Info for CA264_04350 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: alanine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 TIGR00518: alanine dehydrogenase" amino acids 1 to 370 (370 residues), 537.3 bits, see alignment E=9e-166 PF05222: AlaDh_PNT_N" amino acids 4 to 137 (134 residues), 161 bits, see alignment E=3.7e-51 PF01262: AlaDh_PNT_C" amino acids 141 to 352 (212 residues), 306.9 bits, see alignment E=1.3e-95 PF02826: 2-Hacid_dh_C" amino acids 167 to 268 (102 residues), 30.6 bits, see alignment E=4.4e-11 PF00070: Pyr_redox" amino acids 170 to 225 (56 residues), 25.8 bits, see alignment 2.5e-09

Best Hits

Swiss-Prot: 61% identical to DHA_METMP: Alanine dehydrogenase (ald) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K00259, alanine dehydrogenase [EC: 1.4.1.1] (inferred from 80% identity to mtt:Ftrac_3618)

MetaCyc: 57% identical to alanine dehydrogenase subunit (Klebsiella aerogenes)
Alanine dehydrogenase. [EC: 1.4.1.1]

Predicted SEED Role

"Alanine dehydrogenase (EC 1.4.1.1)" in subsystem Pyruvate Alanine Serine Interconversions (EC 1.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.1

Use Curated BLAST to search for 1.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YPC6 at UniProt or InterPro

Protein Sequence (370 amino acids)

>CA264_04350 alanine dehydrogenase (Pontibacter actiniarum KMM 6156, DSM 19842)
MIIGVPKEIKNNENRVGATPAGVIELVRNGHTVYVQSTAGEGSGFMDDDYTAAGATILPS
IEEVYGIADMIIKVKEPIESEYNLIKENQLLFTYFHFASHEPLTNAMIERKAVCLAYETV
EKADRTLPLLVPMSEVAGRMSVQEGAKYLEKPLKGRGILLGGVPGVRPAKVMILGGGVVG
TNAAKMAAGMGADVTILDNNLQRLRYLDDIMPANVNTFMSNEYNIRELLPTHDLIIGAVL
IPGAKAPHLITRDMLKEMRPGTVVVDVAVDQGGCIETCKPTTHQDPTYVIDDVLHYCVAN
MPGAVPYTSTLALTNATLPYALMIANKGWKKACADNAELRKGLNVVHGKVVYPGVAEAFG
LEYTPVESIL