Protein Info for CA264_04250 in Pontibacter actiniarum KMM 6156, DSM 19842
Annotation: NADH oxidoreductase (quinone) subunit F
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to NUOF_SALTY: NADH-quinone oxidoreductase subunit F (nuoF) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 73% identity to cpi:Cpin_3821)MetaCyc: 59% identical to NADH:quinone oxidoreductase subunit F (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)
MetaCyc Pathways
- aerobic respiration I (cytochrome c) (3/4 steps found)
- aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
- NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
- NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
- Fe(II) oxidation (2/6 steps found)
- NAD(P)/NADPH interconversion (2/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.6.5.3
Use Curated BLAST to search for 1.6.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9YPA7 at UniProt or InterPro
Protein Sequence (433 amino acids)
>CA264_04250 NADH oxidoreductase (quinone) subunit F (Pontibacter actiniarum KMM 6156, DSM 19842) MEKPLTQHIVPGRKPLSLKEYEKVGGYQSVRKVLQDMAPAEVQALVKDANLRGRGGAGFN TGLKWSFVPMGPEAATPKYLVANADEMEPGTFKDRLLLEGNPHQLIEGMIVAAYAIQASI SYVFLRWAYKLAAQEITQAIHEAYKAGYLGKNILGTGFSLEMHLHTGVGRYMCGEETALL NALEGKRANPRAKPPFPQVSGLFGKPTIVNNVETLCNLPHIINNGAEWFRKLGLTEDAGT KLYGVSGRVKKPGCWELPMGTTIREILEDYAGGMQDGYLFKGLLPGGASTDFLVEEHLDL PMDYGAIQAAGSRMGTGTMIILDDQTCPVGFVHNLQHFFAQESCGFCTPCREGLPWVEKI LLAIDKGEGKPEHLLTLDFHTKYLGPGNTFCALAPGAMEPLQSALKYFREDFERHIHEHH CPWSKSKAWQPSI