Protein Info for CA264_02370 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: O-acetylhomoserine aminocarboxypropyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 6 to 430 (425 residues), 682.9 bits, E=7.1e-210
• PF01053: Cys_Met_Meta_PP
  • amino acids 8 to 429 (422 residues), 457.3 bits, E=4.9e-141
• PF01041: DegT_DnrJ_EryC1
  • amino acids 60 to 185 (126 residues), 39.5 bits, E=6.6e-14
• PF00155: Aminotran_1_2
  • amino acids 64 to 223 (160 residues), 31.7 bits, E=1.5e-11

Best Hits

Swiss-Prot: 65% identical to CYSD_EMENI: Homocysteine synthase (cysD) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 82% identity to chu:CHU_0274)

MetaCyc: 56% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (11/15 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• aspartate superpathway (17/25 steps found)
• L-methionine biosynthesis II (3/6 steps found)
• L-methionine biosynthesis I (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See A0A1X9YNF4 at UniProt or InterPro

Protein Sequence (439 amino acids)

>CA264_02370 O-acetylhomoserine aminocarboxypropyltransferase (Pontibacter actiniarum KMM 6156, DSM 19842)
MSKSLHFETLQLHAGQEIDPTTQSRAVPIYQTTSYAFKNAEHGANLFALKEFGNIYTRIM
NPTTDVFEKRIAALEGGVAAVAVASGQAAQFIALTNILEAGDNFITTSYLYGGTYNQFKV
SFKRLGIETRFAETDEVASYEKLIDEKTRAIYVETIGNPRFNIPDFEAVAALARKYDIPL
VVDNTFGAGGYLFRPLEHGANVVVESATKWIGGHGTSIGGVLVDGGNFNWGNGKFKQFSE
PSEGYHGLNFWEVFGEGSPFGNIAFAIRARVEGLRDFGPALSPFNSFLLLQGLETLSLRV
DRTVQNALALAQWLERQELVESVNYPGLESSPYHELAQKYLKRGFGGVLSFKLKGDKQQA
EKFVNSLQLVSHLANVGDAKTLIIHPASTTHQQLSDAEQRSAGVDPTLLRISAGIEHLDD
IKADFEQAFAKVEAEPAIA