Protein Info for CA264_02060 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 843 PF17764: PriA_3primeBD" amino acids 23 to 120 (98 residues), 98.7 bits, see alignment E=4.3e-32 PF04851: ResIII" amino acids 304 to 467 (164 residues), 50.7 bits, see alignment E=6.1e-17 PF00270: DEAD" amino acids 308 to 470 (163 residues), 71 bits, see alignment E=3.1e-23 TIGR00595: primosomal protein N'" amino acids 328 to 843 (516 residues), 582.2 bits, see alignment E=4.3e-179 PF18319: PriA_CRR" amino acids 557 to 583 (27 residues), 43.1 bits, see alignment (E = 9.6e-15) PF00271: Helicase_C" amino acids 607 to 701 (95 residues), 40.3 bits, see alignment E=9.9e-14 PF18074: PriA_C" amino acids 744 to 842 (99 residues), 38 bits, see alignment E=7.2e-13

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 55% identity to dfe:Dfer_3377)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YN86 at UniProt or InterPro

Protein Sequence (843 amino acids)

>CA264_02060 primosomal protein N' (Pontibacter actiniarum KMM 6156, DSM 19842)
MSELLNFNYPPEPATDRVTLFADVILPLPLPKLYTYRIPFEMNDEVLVGARVIVQFGSKK
VLSCIVADIHENAPADYQAKYVLDVLDDKPIVTAPQLKLFKWIADYYMCTLGEVINAALP
SALKLSSESRIQLHPHYNPEEDELVLAAQEEKIIFALQNNQALTFSEVGNLLQLKSYHKF
IKSLIQKEAIIIFEQVSDRFSPKVVKKVRLSEHFVQEEGMLEELFNQLAPQPKQLDVLLN
YLQLVPVHQDIHLNYMGVDKAALTNNPHLSASSINTLIKKGVLEQFDKIVSRFPMDHGTQ
QLPMALSEHQLQAKDEILGLFKEKDTVLLHGVTGSGKTEVYIDLIKHALDSGGQVLYLLP
EIALTAQIVTRLMKVFGDKLGVYHSKFSDNERAEVWQGILSGRFQVVVGVRSSVFLPFHD
LSLVIIDEEHEASYKQYEPAPRYNARETGLMMAHLQGAKTLLGSATPSIESYYNCKTGRW
GLVSMTKRFGEARLPDIELVDTRREQQRKNMHSHFSAKLLQDIEERLKRHEQVILFQNRR
GYAPFISCDECSWIPKCKFCAVSLSYHKFNNELRCHYCGYHERMPHDCPACGATTLRSMG
FGTEKVEDELKLLLPDAEVQRMDLDTTKKKNSYQQIISDFESGRTNVLVGTQMVTKGLDF
ENVSLVGILNADTIINFPDFRAHERAYQLFVQVSGRAGRKGKPGSVIIQTRDPLQPIFNK
VRSNDYQTLYEHEIEERMRFGYPPFTRMIRVTVKHADDKIAENAAIVLAKDLTDRLGKQQ
VLGPEVPYIFKIRNLFLQEIHIKLPRDTTNLKAAKGQIAQAIFNLKLLSDFKGIRVVADV
DPV