Protein Info for CA264_02005 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: 23S rRNA (uracil-5-)-methyltransferase RumA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01938: TRAM
  • amino acids 16 to 64 (49 residues), 28 bits, 4.1e-10
• TIGR00479: 23S rRNA (uracil-5-)-methyltransferase RumA
  • amino acids 24 to 462 (439 residues), 347.1 bits, E=7e-108
• PF02475: Met_10
  • amino acids 225 to 391 (167 residues), 27.8 bits, E=5.4e-10
• PF05958: tRNA_U5-meth_tr
  • amino acids 291 to 469 (179 residues), 72.7 bits, E=7.2e-24
• PF13847: Methyltransf_31
  • amino acids 324 to 401 (78 residues), 39.9 bits, E=9.1e-14
• PF09445: Methyltransf_15
  • amino acids 326 to 407 (82 residues), 24.4 bits, E=4.8e-09

Best Hits

Swiss-Prot: 62% identical to Y643_BACTN: Uncharacterized RNA methyltransferase BT_0643 (BT_0643) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00599, [EC: 2.1.1.-] (inferred from 62% identity to mtt:Ftrac_0805)

Predicted SEED Role

"RNA methyltransferase, TrmA family"

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See A0A1X9YN75 at UniProt or InterPro

Protein Sequence (470 amino acids)

>CA264_02005 23S rRNA (uracil-5-)-methyltransferase RumA (Pontibacter actiniarum KMM 6156, DSM 19842)
MRNKSKKKKFEILEHIKIEDMVAEGKCLARHDNMVIFVAGVAPGDVVDLRITRKKKSFLE
AEPVAFHELSELRVQPFCEHFGTCGGCKWQHIGYDTQLHFKQKQVKDNLERIGKVPLPAF
DPILGSANTRYYRNKLEYTFSNTSWLTKEQIQSGQEFDRDALGFHIPGRFDKILDIKHCY
LQPDPSNAIRLEVRDYAKKHDLPFFDIVKQEGYMRNLIIRTANTGEVMVIVQVYEDRPEE
MEALMQHLAATFPEITSLQYVVNSKGNETFHDLEVVCYKGLPYIHEDMEGIKFRVGPKSF
YQTNADQAYELYKKTRELANLTGNELVYDLYTGAGTIANFVAHQAREVIGIEYVPSAIED
AKINSQINNITNTTFYAGDMKDILSDELIERHGRPDVVITDPPRAGMHEDVVKKLLQVHP
NRIVYVSCNPATQARDVEWLSEKYDVTRVQPVDMFPQTHHVENIVLLTAR