Protein Info for CA264_00760 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: NAD(+) synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 8 to 248 (241 residues), 78.9 bits, E=4.1e-26
• PF02540: NAD_synthase
  • amino acids 288 to 567 (280 residues), 99.9 bits, E=1.3e-32
• TIGR00552: NAD+ synthetase
  • amino acids 290 to 597 (308 residues), 86.2 bits, E=1e-28

Best Hits

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 56% identity to chu:CHU_2417)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• NAD de novo biosynthesis II (from tryptophan) (9/9 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• superpathway of NAD biosynthesis in eukaryotes (11/14 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway V (PNC V cycle) (4/5 steps found)
• NAD de novo biosynthesis I (4/6 steps found)
• NAD de novo biosynthesis III (4/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (4/6 steps found)
• aspartate superpathway (17/25 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Sequence Analysis Tools

See A0A1X9YMH8 at UniProt or InterPro

Protein Sequence (628 amino acids)

>CA264_00760 NAD(+) synthase (Pontibacter actiniarum KMM 6156, DSM 19842)
MEETRLMLAAAALNQTPMDWAGNLQNIQTAINEAKQNNTDLLLLPELAITGYGCEDMFLS
PWLSEMAFNQLLQVKDMCDGITVAVGLPVFLNMRVYNTACVIRNKEIIGFTAKQFLANDG
VHYEPRWFTPWPANEVQEFEMLGQKYSIGDIVYEEQGVKYAFEICEDAWRPNRPAERHMP
KGVQLILNPSASHFALSKTDVRYRLVVDASKKYQCAYMYANLLGNEAGRMIYDGEVLIAQ
NGKLIRRNELLCFKDVDMECAEVCFSDNPEIAEVIEYLPPIDENKEVIAALSLALFDYMR
KSRSRGFVLSLSGGADSSLCAVAVAEMVRRGVESLGREAFVEKALFFSAEDKARIAQLPQ
EEAQKAIVNKLLTCAYQGTVNSSDDTYTSAKELADSIGARFYNWTIDEEVKGYIGKIEHA
LGRKLTWEQDDITLQNIQARVRAPGIWMLANIQYALLMATSNRSEASVGYATMDGDTAGS
ISPIAGIDKAFIRQFLVWAQQELGYTGLQYVNNLQPSAELRPSEEEQTDEKDLMPYEVLN
QIERLAFHDRYSPEEVYTILLEQQVAQAEVLKAWITKFYSLWSRNQWKRERYAPAFHLDD
YNVDPRSWLRFPILSGGFREELKRVQDK