Protein Info for CA264_00570 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 679 PF00133: tRNA-synt_1" amino acids 3 to 248 (246 residues), 48.6 bits, see alignment E=1.1e-16 PF09334: tRNA-synt_1g" amino acids 9 to 397 (389 residues), 529.1 bits, see alignment E=1.2e-162 TIGR00398: methionine--tRNA ligase" amino acids 9 to 542 (534 residues), 548.9 bits, see alignment E=1.3e-168 PF19303: Anticodon_3" amino acids 409 to 551 (143 residues), 76.9 bits, see alignment E=3.1e-25 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 540 to 678 (139 residues), 124.8 bits, see alignment E=2.3e-40 PF01588: tRNA_bind" amino acids 584 to 675 (92 residues), 85.1 bits, see alignment E=5.8e-28

Best Hits

Swiss-Prot: 71% identical to SYM_CYTH3: Methionine--tRNA ligase (metG) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 72% identity to osp:Odosp_3293)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YMF9 at UniProt or InterPro

Protein Sequence (679 amino acids)

>CA264_00570 methionine--tRNA ligase (Pontibacter actiniarum KMM 6156, DSM 19842)
MTNNPKRYTVTAALPYANGPVHIGHLAGVYLPADIYVRYLRLQNRDVKFICGSDEHGVPI
TIRAKKEGITPQQVVDKYHELIRDSFKDFNISFDIYDRTSSEIHAETAGDFFKKLYEDGK
FIEQTTQQYYDEKAQQFLADRYIVGTCPKCGNENAYGDQCEACGTSLNATDLINPKSTLS
GAVPVMRETKHWYLPLNEYEPWLREWIVEGHKGDWKPNVYGQCKSWIDQGLQPRAVTRDL
DWGVPVPVEGAEGKVLYVWFDAPIGYISATKALTDDWEKYWKDEESKLVHFIGKDNIVFH
CIIFPSMLKAHGDYILPDNVPANEFLNLEGDKISTSRNWAVWLHEYLQDFPGKGDVLRYV
LAANAPETKDNDFTWKDYQARNNNELLAILGNFINRAVVLTQKYYEGAVPARGELTEYDK
EVLGVLEGMPLVIASYIERYRFRDALGELMNLARLGNKYLADTEPWKLIKTDAERVKTIM
NIALQISGSLAILMEPFLPDSAAKLRGMLSMNPAMWADAGAANLLPAGHIIGKPELLFEK
IEDTAVDAQVQKLLDTKKANELANATVEPAKENITFDDFTKLDVRVGTIIEAEKVAKTKK
LLKLKVDTGIDQRTVVSGIAEFFKPEDIVGQQVSILVNLAPRDIKGITSQGMILMAENAD
GSLAFVQPSKQITNGGTVS