Protein Info for BNILDI_18285 in Escherichia coli ECRC62

Name: puuP
Annotation: putrescine/proton symporter PuuP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 24 to 48 (25 residues), see Phobius details amino acids 57 to 77 (21 residues), see Phobius details amino acids 98 to 121 (24 residues), see Phobius details amino acids 133 to 150 (18 residues), see Phobius details amino acids 162 to 182 (21 residues), see Phobius details amino acids 202 to 221 (20 residues), see Phobius details amino acids 241 to 264 (24 residues), see Phobius details amino acids 288 to 311 (24 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 365 to 386 (22 residues), see Phobius details amino acids 398 to 417 (20 residues), see Phobius details amino acids 423 to 442 (20 residues), see Phobius details PF00324: AA_permease" amino acids 27 to 416 (390 residues), 140.2 bits, see alignment E=8.9e-45 PF13520: AA_permease_2" amino acids 60 to 418 (359 residues), 103.6 bits, see alignment E=1.2e-33

Best Hits

Swiss-Prot: 100% identical to PUUP_ECOLI: Putrescine importer PuuP (puuP) from Escherichia coli (strain K12)

KEGG orthology group: K14052, putrescine importer (inferred from 100% identity to eco:b1296)

MetaCyc: 100% identical to putrescine:H+ symporter PuuP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-69

Predicted SEED Role

"Putrescine importer"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>BNILDI_18285 putrescine/proton symporter PuuP (Escherichia coli ECRC62)
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYEGDRTPVSET