Protein Info for BNILDI_17110 in Escherichia coli ECRC62

Name: ydfJ
Annotation: MHS family MFS transporter YdfJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 54 to 77 (24 residues), see Phobius details amino acids 89 to 109 (21 residues), see Phobius details amino acids 115 to 136 (22 residues), see Phobius details amino acids 155 to 180 (26 residues), see Phobius details amino acids 188 to 208 (21 residues), see Phobius details amino acids 251 to 270 (20 residues), see Phobius details amino acids 282 to 300 (19 residues), see Phobius details amino acids 308 to 328 (21 residues), see Phobius details amino acids 335 to 356 (22 residues), see Phobius details amino acids 377 to 396 (20 residues), see Phobius details amino acids 402 to 422 (21 residues), see Phobius details PF07690: MFS_1" amino acids 21 to 344 (324 residues), 104.9 bits, see alignment E=6.8e-34 amino acids 256 to 432 (177 residues), 38 bits, see alignment E=1.5e-13 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 23 to 418 (396 residues), 462.7 bits, see alignment E=5.7e-143 PF00083: Sugar_tr" amino acids 57 to 219 (163 residues), 76.9 bits, see alignment E=2.4e-25 PF13347: MFS_2" amino acids 151 to 362 (212 residues), 27.3 bits, see alignment E=2.1e-10

Best Hits

Swiss-Prot: 100% identical to YDFJ_ECOLI: Putative transporter YdfJ (ydfJ) from Escherichia coli (strain K12)

KEGG orthology group: K08173, MFS transporter, MHS family, metabolite:H+ symporter (inferred from 100% identity to ecp:ECP_1527)

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>BNILDI_17110 MHS family MFS transporter YdfJ (Escherichia coli ECRC62)
MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHEIFFPESSTAMALILAM
GTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPIL
LVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFI
LSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQS
KSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWL
SDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENIT
MAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLIS
ALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL