Protein Info for BNILDI_15600 in Escherichia coli ECRC62

Annotation: alpha-glycosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 PF02903: Alpha-amylase_N" amino acids 4 to 144 (141 residues), 85.7 bits, see alignment E=2.9e-28 PF00128: Alpha-amylase" amino acids 195 to 497 (303 residues), 255.7 bits, see alignment E=9e-80

Best Hits

KEGG orthology group: None (inferred from 100% identity to ecr:ECIAI1_1931)

MetaCyc: 66% identical to cyclodextrinase (Klebsiella michiganensis M5al)
Cyclomaltodextrinase. [EC: 3.2.1.54]

Predicted SEED Role

"Neopullulanase (EC 3.2.1.135)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.135)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.135 or 3.2.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (599 amino acids)

>BNILDI_15600 alpha-glycosidase (Escherichia coli ECRC62)
MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGDPYNWAEGGLDGGNMAG
TDAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFILFGKEGEKFLFGEKRCVD
ISSPECEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVWYQIFPDRFCNGRPEISPEGV
EPWGSTPTSFNFMGGDLWGVIDKLDYLEDLGIGGIYFCPIFTSASNHKYDTIDHFSVDPH
LGGNIAFHTLIEEAHKLGIKIMLDAVFNHLGADSPIWLDVVRNGANSRYADWFWIHKFPV
YPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEECREYLLSIVRYWTQNFDIDGWRLDVA
NEVDHHFWRDFRKVIKDIKPECYILGEIWHEGTPWLRGDQFDSLMNYPLTYGIIDYFALQ
DTTKQEFMTSVTRSYLCYPKNITEVMFNLLDSHDTARILSVCLNDKRKVKLAYLFMLTQA
GSPCIYYGSEIGIDGFKSMTLENNRKCMIWDENKQDLELRQFIRWLIRLRKKHPQWCEAS
IQWKDVEHPTVIAYQRDNITFFLNNSEDTANFIYDGRSMEISGFSYEIEGLPAADLYDF