Protein Info for BNILDI_14875 in Escherichia coli ECRC62

Name: cobT
Annotation: nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02277: DBI_PRT
  • amino acids 11 to 345 (335 residues), 402.5 bits, E=7.5e-125
• TIGR03160: nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
  • amino acids 15 to 346 (332 residues), 391.2 bits, E=2.1e-121

Best Hits

Swiss-Prot: 100% identical to COBT_ECO57: Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) from Escherichia coli O157:H7

KEGG orthology group: K00768, nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase [EC: 2.4.2.21] (inferred from 100% identity to ecz:ECS88_2056)

MetaCyc: 79% identical to nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase. [EC: 2.4.2.21]; 2.4.2.21 [EC: 2.4.2.21]; 2.4.2.21 [EC: 2.4.2.21]

Predicted SEED Role

"Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.4.2.21)

MetaCyc Pathways

• superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
• 2-methyladeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• 5-hydroxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• 5-methoxy-6-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• 5-methoxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• 5-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• adeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• adenosylcobalamin biosynthesis from adenosylcobinamide-GDP I (3/3 steps found)
• benzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (7/9 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (16/36 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism
• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.21

Protein Sequence (359 amino acids)

>BNILDI_14875 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (Escherichia coli ECRC62)
MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGVQLAGMPGLNGIPHVGK
KAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVLAAQAGANVHVIDVGIDTA
EPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYTRELAKNGVTLFGVGELGMAN
TTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVVRRAITLNQPNPQDGVNVLAKVG
GFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAACQMSPAIKPYLIPSHLSAEKGARIA
LSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACAIYNNMGELAASKIVLPGNTTSDLNS