Protein Info for BNILDI_10630 in Escherichia coli ECRC62

Name: relA
Annotation: GTP diphosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 PF13328: HD_4" amino acids 43 to 191 (149 residues), 143.1 bits, see alignment E=2e-45 TIGR00691: RelA/SpoT family protein" amino acids 63 to 739 (677 residues), 619.2 bits, see alignment E=4.7e-190 PF04607: RelA_SpoT" amino acids 251 to 360 (110 residues), 144.5 bits, see alignment E=4.4e-46 PF02824: TGS" amino acids 406 to 465 (60 residues), 78.1 bits, see alignment 1.3e-25 PF19296: RelA_AH_RIS" amino acids 483 to 645 (163 residues), 55.4 bits, see alignment E=2.8e-18 PF13291: ACT_4" amino acids 665 to 740 (76 residues), 65.1 bits, see alignment E=2.3e-21 PF01842: ACT" amino acids 668 to 733 (66 residues), 38.5 bits, see alignment E=2.3e-13

Best Hits

Swiss-Prot: 100% identical to RELA_ECO57: GTP pyrophosphokinase (relA) from Escherichia coli O157:H7

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to ecp:ECP_2765)

MetaCyc: 100% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (744 amino acids)

>BNILDI_10630 GTP diphosphokinase (Escherichia coli ECRC62)
MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQQTQGHPDASLLLWRGV
EMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKSVVNLIHGVRDMAAIRQLK
ATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHLREVKDAPEDERVLAAKECTN
IYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLLHERRLDREHYIEEFVGHLRAEM
KAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDVRAVRIVAERLQDCYAALGIVHTHY
RHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTVEIQIRTKQMHEDAELGVAAHWKYKEG
AAAGGARSGHEDRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVVDLPA
GSTPLDFAYHIHSDVGHRCIGAKIGGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNL
GYVTTSRGRSKIHAWFRKQDRDKNILAGRQILDDELEHLGISLKEAEKHLLPRYNFNDVD
ELLAAIGGGDIRLNQMVNFLQSQFNKPSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVE
GVGNLMHHIARCCQPIPGDEIVGFITQGRGISVHRADCEQLAELRSHAPERIVDAVWGES
YSAGYSLVVRVVANDRSGLLRDITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQ
VLGRVLGKLNQVPDVIDARRLHGS