Protein Info for BNILDI_10415 in Escherichia coli ECRC62

Name: recB
Annotation: exodeoxyribonuclease V subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1180 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 8 to 1172 (1165 residues), 1547.2 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 15 to 436 (422 residues), 179.1 bits, see alignment E=3.8e-56 PF13361: UvrD_C" amino acids 441 to 808 (368 residues), 60.3 bits, see alignment E=5.9e-20 PF12705: PDDEXK_1" amino acids 950 to 1157 (208 residues), 24.1 bits, see alignment E=8.3e-09

Best Hits

Swiss-Prot: 100% identical to RECB_ECOLI: RecBCD enzyme subunit RecB (recB) from Escherichia coli (strain K12)

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 100% identity to ecw:EcE24377A_3140)

MetaCyc: 100% identical to exodeoxyribonuclease V subunit RecB (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]; Exodeoxyribonuclease V. [EC: 5.6.2.4, 3.1.11.5]; RXN-19004 [EC: 5.6.2.4, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1180 amino acids)

>BNILDI_10415 exodeoxyribonuclease V subunit beta (Escherichia coli ECRC62)
MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGLGGSAAFPRPLTVEELL
VVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLEEIDDKAQAAQWLLLAERQ
MDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLRYQACADFWRRHCYPLPREIA
QVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETLASRHAQIVARIDTVKQQWRDAV
GELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEETNSYQLPESLEKFSQRFLEDRTKA
GGETPRHPLFEAIDQLLAEPLSIRDLVITRALAEIRETVAREKRRRGELGFDDMLSRLDS
ALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQQYRIFRRIWHHQPETALLLIGDPKQAIY
AFRGADIFTYMKARSEVHAHYTLDTNWRSAPGMVNSVNKLFSQTDDAFMFREIPFIPVKS
AGKNQALRFVFKGETQPAMKMWLMEGESCGVGDYQSTMAQVCAAQIRDWLQAGQRGEALL
MNGDDARPVRASDISVLVRSRQEAAQVRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLL
QAVMTPERENTLRSALATSMMGLNALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPM
LRALMSARNIAENLLATAGGERRLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDS
NASSQQMRLESDKHLVQIVTIHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDL
NAAPESVDLAEAERLAEDLRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGR
LLQKGEPQDAAGLRTCIEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDN
WRVTSYSGLQQRGHGIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFE
DLDFTQPVDPNWVREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVE
MEFYLPISEPLIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLD
YKSNWLGEDSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEHHFGGVIY
LFLRGVDKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA