Protein Info for BNILDI_06475 in Escherichia coli ECRC62

Name: ccmF
Annotation: Cytochrome c biogenesis protein CcmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 540 signal peptide" amino acids 9 to 19 (11 residues), see Phobius details transmembrane" amino acids 20 to 34 (15 residues), see Phobius details amino acids 66 to 85 (20 residues), see Phobius details amino acids 92 to 114 (23 residues), see Phobius details amino acids 146 to 166 (21 residues), see Phobius details amino acids 178 to 200 (23 residues), see Phobius details amino acids 220 to 236 (17 residues), see Phobius details amino acids 247 to 273 (27 residues), see Phobius details amino acids 282 to 301 (20 residues), see Phobius details amino acids 321 to 345 (25 residues), see Phobius details amino acids 364 to 382 (19 residues), see Phobius details amino acids 389 to 410 (22 residues), see Phobius details amino acids 515 to 533 (19 residues), see Phobius details TIGR00353: cytochrome c-type biogenesis protein CcmF" amino acids 24 to 378 (355 residues), 605.5 bits, see alignment E=6.6e-186 PF01578: Cytochrom_C_asm" amino acids 60 to 266 (207 residues), 169.5 bits, see alignment E=8.4e-54 PF16327: CcmF_C" amino acids 289 to 391 (103 residues), 76.7 bits, see alignment E=2.1e-25 amino acids 391 to 531 (141 residues), 125.7 bits, see alignment E=2.6e-40

Best Hits

Swiss-Prot: 98% identical to NRFE_ECOLI: Probable cytochrome c-type biogenesis protein NrfE (nrfE) from Escherichia coli (strain K12)

KEGG orthology group: K04016, formate-dependent nitrite reductase, possible assembly protein (inferred from 99% identity to eoh:ECO103_4825)

Predicted SEED Role

"Cytochrome c-type heme lyase subunit nrfE, nitrite reductase complex assembly" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (540 amino acids)

>BNILDI_06475 Cytochrome c biogenesis protein CcmF (Escherichia coli ECRC62)
LRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVW
GGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVR
IFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVVASVALASLLRGEFDGACARICWR
WALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRE
IFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALY
GWRARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEVSLNLQ
PGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQQMME
PSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGKKRDE