Protein Info for BNILDI_06065 in Escherichia coli ECRC62

Name: purH
Annotation: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 8 to 529 (522 residues), 906.1 bits, E=3.5e-277
• PF02142: MGS
  • amino acids 19 to 132 (114 residues), 99.7 bits, E=8.5e-33
• PF01808: AICARFT_IMPCHas
  • amino acids 137 to 459 (323 residues), 431.5 bits, E=2.2e-133

Best Hits

Swiss-Prot: 100% identical to PUR9_ECO24: Bifunctional purine biosynthesis protein PurH (purH) from Escherichia coli O139:H28 (strain E24377A / ETEC)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 99% identity to eco:b4006)

MetaCyc: 99% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Protein Sequence (529 amino acids)

>BNILDI_06065 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (Escherichia coli ECRC62)
MQQRRPIRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVVVNLYPFAKTVAREGCSLE
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEMDDNEGSLTLATRFDLAIKAF
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAISNSI
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTQRQPTEQELRDALFC
WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH