Protein Info for BNILDI_04465 in Escherichia coli ECRC62

Annotation: alpha/beta hydrolase-fold protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02922: CBM_48" amino acids 44 to 103 (60 residues), 34.2 bits, see alignment E=2.6e-12 PF00756: Esterase" amino acids 150 to 297 (148 residues), 55 bits, see alignment E=1e-18

Best Hits

Predicted SEED Role

"Endo-1,4-beta-xylanase A precursor (EC 3.2.1.8)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Xylose utilization (EC 3.2.1.8)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (317 amino acids)

>BNILDI_04465 alpha/beta hydrolase-fold protein (Escherichia coli ECRC62)
MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVNADNSVTFRYFAPGAKN
VSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNVDGVRSIDTGTAMTKPQRQ
VNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPPGYTGMGEPLPVLYFY
HGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDAKGIIPEDFVPQERRKVF
YPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQGGYQALVSGMNHLESFGWPH
SVVLPRQPYRMKVSRPG