Protein Info for BNILDI_02460 in Escherichia coli ECRC62

Name: yheT
Annotation: hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF12146: Hydrolase_4" amino acids 70 to 286 (217 residues), 41.4 bits, see alignment E=1.6e-14 PF00561: Abhydrolase_1" amino acids 73 to 313 (241 residues), 142.7 bits, see alignment E=2.4e-45

Best Hits

Swiss-Prot: 99% identical to YHET_ECOLI: Putative esterase YheT (yheT) from Escherichia coli (strain K12)

KEGG orthology group: K07019, (no description) (inferred from 99% identity to eco:b3353)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>BNILDI_02460 hydrolase (Escherichia coli ECRC62)
MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFTPYWQRLELPDGDFVDL
AWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGWLGVVMHFRGCSGEPNRMH
RIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLACLLAKEGNDLPVDAAVIVSA
PFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAYPGTLPINLAQLKSVRRIREFDD
LITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIHAKDDPFMDHQVIPKPESLPPQVEY
QLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTYLEAKSC