Protein Info for BNILDI_00065 in Escherichia coli ECRC62
Name: aRA1
Annotation: aldo/keto reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to YTBE_BACSU: Uncharacterized oxidoreductase YtbE (ytbE) from Bacillus subtilis (strain 168)
KEGG orthology group: None (inferred from 98% identity to ecm:EcSMS35_0333)MetaCyc: 47% identical to glyoxal/methylglyoxal reductase (Bacillus subtilis subtilis 168)
Aryl-alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.91]; 1.1.1.91 [EC: 1.1.1.91]; Methylglyoxal reductase (NADPH-dependent). [EC: 1.1.1.91, 1.1.1.283]
Predicted SEED Role
"2,5-diketo-D-gluconic acid reductase (EC 1.1.1.-)" (EC 1.1.1.-)
MetaCyc Pathways
- superpathway of methylglyoxal degradation (7/8 steps found)
- methylglyoxal degradation IV (3/3 steps found)
- methylglyoxal degradation V (3/3 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Polyketide sugar unit biosynthesis
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-
Use Curated BLAST to search for 1.1.1.- or 1.1.1.283 or 1.1.1.91
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (289 amino acids)
>BNILDI_00065 aldo/keto reductase (Escherichia coli ECRC62) VEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDAVGEAVR EAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMRDYFS AWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEALATMKY YNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRI EENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD