Protein Info for BACOVA_04582 in Bacteroides ovatus ATCC 8483

Annotation: iron-only hydrogenase maturation rSAM protein HydG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 TIGR03955: [FeFe] hydrogenase H-cluster radical SAM maturase HydG" amino acids 3 to 473 (471 residues), 942.8 bits, see alignment E=1.3e-288 PF04055: Radical_SAM" amino acids 93 to 244 (152 residues), 58 bits, see alignment E=1.4e-19 PF06968: BATS" amino acids 276 to 382 (107 residues), 65.2 bits, see alignment E=5e-22

Best Hits

KEGG orthology group: K03150, thiamine biosynthesis ThiH (inferred from 92% identity to bth:BT_1836)

Predicted SEED Role

"2-iminoacetate synthase (ThiH) (EC 4.1.99.19)" (EC 4.1.99.19)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 4.1.99.19

Use Curated BLAST to search for 4.1.99.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (473 amino acids)

>BACOVA_04582 iron-only hydrogenase maturation rSAM protein HydG (Bacteroides ovatus ATCC 8483)
MIYQKDSSKAEEFIHHEEILDTLEYAQNNKDNRVLIEQLIEKAALCKGLTHREAAILLEC
NQPDLIERIFHLAKEIKQKFYGNRIVMFAPLYLSNYCVNGCLYCPYHAKNKTIARKKLTQ
EEIRREVIALQDMGHKRLALEAGEHPSLNPIEYILESIQTIYSIKHKNGAIRRVNVNIAA
TTVENYRRLKEAGIGTYILFQETYHKDNYEALHPTGPKSNYAYHTEAMDRAMEGGIDDVG
IGVLFGLNTYRYDFIGLLMHAEHLEAKFGVGPHTISVPRICSADDINAGDFPNSISDEIF
SKIVAVIRIAVPYTGMIISTRESQESRKKVLELGISQISGGSRTSVGGYAETELPDHNSA
QFDVSDTRTLDEVVNWLLELGYIPSFCTACYREGRTGDRFMSLVKSGQIANCCGPNALMT
LKEYLEDYASEDTRQKGLKLILKETDRIPNPKIREIAIRNLKAIAAGQRDFRF