Protein Info for BACOVA_04507 in Bacteroides ovatus ATCC 8483

Annotation: glycosyl hydrolase family 32

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 626 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF16352: DUF4980" amino acids 39 to 138 (100 residues), 122.2 bits, see alignment E=1.3e-39 PF00251: Glyco_hydro_32N" amino acids 139 to 440 (302 residues), 308.8 bits, see alignment E=8e-96 PF08244: Glyco_hydro_32C" amino acids 448 to 622 (175 residues), 115.3 bits, see alignment E=4.1e-37

Best Hits

KEGG orthology group: K01212, levanase [EC: 3.2.1.65] (inferred from 86% identity to bth:BT_1765)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.65

Use Curated BLAST to search for 3.2.1.65 or 3.2.1.B3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (626 amino acids)

>BACOVA_04507 glycosyl hydrolase family 32 (Bacteroides ovatus ATCC 8483)
MKTNPWMKLCKGAILALAVSYGLTYCHTTKSKLTLEQKGDSLTVIHITNPTNYILLPIEE
EAAESQVLLDTGEAADTDMGIRLAQTQVDYFVPFALPVGAKTATVRIRNKSKEALCWKEI
KLSDTFDTTNTEKFRPVYHHTPLYGWMNDANGLVYKDGEYHLYFQYNPYGSKWGNMHWGH
SVSKDLVHWEHLAPAIARDTLGHIFSGSSIVDQENIAGYGAGSILAFYTSASDKNGQIQC
LAFSKDNGRTFTKYEKNPILCPADGLKDFRDPKVFRYEPEDKWVMIVSADKEMRFYDSKN
LKDWNYMSSFGEGYGVQPCQFECPDMVELPVDGDLNRKKWALIVNVNPGCYFGGSATQYF
TGDFDGKKFSCDNQPNVTKWLDWGKDHYATVCFSNTGERTIAVPWMSNWQYCNIVPTKQF
RSANALPRELSLYTQDGEIYLSAAPVLEIKTLRKEKKETPAFTVANDYHIDSLLADNDGA
YELALEITAGEAEIMGFSLFNDKGEKVDIYFNLPEKRLVMDRTKSGIVDFGKKSVPHEIE
VHDRRKTTSINYIDDFALATWAPIKKENKYTLDVFVDKCSVEIFLNGGKVAMTNLIFPSA
PYNRMCFYSKGGSFQVDSFNAYRLGL