Protein Info for BACOVA_04453 in Bacteroides ovatus ATCC 8483
Annotation: Biotin-requiring enzyme
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01960, pyruvate carboxylase subunit B [EC: 6.4.1.1] (inferred from 94% identity to bth:BT_1697)Predicted SEED Role
"Oxaloacetate decarboxylase alpha chain (EC 4.1.1.3)" in subsystem Na+ translocating decarboxylases and related biotin-dependent enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.3)
MetaCyc Pathways
- gluconeogenesis III (10/12 steps found)
- reductive TCA cycle I (9/11 steps found)
- incomplete reductive TCA cycle (6/7 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
- methylgallate degradation (2/6 steps found)
- protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
- superpathway of vanillin and vanillate degradation (3/10 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
- syringate degradation (3/12 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Citrate cycle (TCA cycle)
- Pyruvate metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.1.1.3, 6.4.1.1
Use Curated BLAST to search for 4.1.1.3 or 6.4.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (611 amino acids)
>BACOVA_04453 Biotin-requiring enzyme (Bacteroides ovatus ATCC 8483) MKREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGE NPNKAVREWTKPFHEAGIQTHMLDRALNGLRMSPVPADVRKLFYKVKKAQGTDITRTFCG LNDVRNIAPSITYAKEAGMISQCSLCITHSPIHTVEYYTNMALELIKLGADEICIKDMAG IGRPVSLGKIVANIKAAHPEIPVQYHSHAGPGFNMASILEVCEAGCDYIDVGMEPLSWGT GHADLLSVQAMLKDAGYQVPEINMEAYMKVRGMIQEFMDDFLGLYISPKNRLMNSLLIAP GLPGGMMGSLMADLESNLESINKYKAKHNLPFMTQDQLLIKLFDEVAYVWPRVGYPPLVT PFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMILGKAGRLPGKLAPEIIEKAEREGRK FFEGDPQNNYPDALDKYRKLMKENKWEVGQDDEELFEYAMHPAQYEAYKSGKAKEDFLED VVKRRAEKDKSPEEDAKPKTLTVQVDGQAYRVTVAYGDTELPAAPAGAAAAPAGEGKEVL SPLEGKFFLVKGAQETPLQVGDTVKEGDVICYVEAMKTYNAIRAEFGGTVTAICVNPGDA VSEDDVLMKIG