Protein Info for BACOVA_03030 in Bacteroides ovatus ATCC 8483

Annotation: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 7 to 426 (420 residues), 649.8 bits, E=7.8e-200
• PF01053: Cys_Met_Meta_PP
  • amino acids 9 to 425 (417 residues), 460.6 bits, E=6.2e-142
• PF00155: Aminotran_1_2
  • amino acids 38 to 226 (189 residues), 30.5 bits, E=4.5e-11
• PF01041: DegT_DnrJ_EryC1
  • amino acids 68 to 190 (123 residues), 34.7 bits, E=2.5e-12
• PF00266: Aminotran_5
  • amino acids 100 to 216 (117 residues), 25.6 bits, E=1.2e-09

Best Hits

Swiss-Prot: 52% identical to CYSD_SCHPO: Homocysteine synthase (met17) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 97% identity to bth:BT_2387)

MetaCyc: 51% identical to O-acetylhomoserine sulfhydrylase (Corynebacterium glutamicum ATCC 13032)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• aspartate superpathway (19/25 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• L-methionine biosynthesis II (3/6 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (5/10 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (428 amino acids)

>BACOVA_03030 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (Bacteroides ovatus ATCC 8483)
MATKNLHFETLQVHVGQEQADPATDARAVPIYQTTSYVFHNSAHAAARFGLQDPGNIYGR
LTNSTQGVFEQRVAALEGGVAGLAVASGAAAITYAFENITRAGDHIVAAKTIYGGSYNLL
AHTLPSYGITTTFVDPSDLSNFEKAIQENTKAVFIETLGNPNSNIIDIEAVAEIAHRHKI
PLIIDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGSSLGGVIVDSGKFDWVASGKFPQL
TEPDPCYHGVRFVDAAGPAAYAIRIRAILLRDTGATISPFNAFILLQGLETLSLRVERHV
ENALKVVNFLNNHPKVKKVNHPSLSNHPDHALYQRYFPNGAGSIFTFEVKGGQEEAHRFI
DSLEIFSLLANVADVKSLVIHPASTTHSQLNAQELAEQEIYPGTVRLSIGTEHIDDLIAD
LDQALAKI