Protein Info for BACOVA_02890 in Bacteroides ovatus ATCC 8483
Annotation: dihydrolipoyl dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 37% identical to DLDH1_ARATH: Dihydrolipoyl dehydrogenase 1, mitochondrial (LPD1) from Arabidopsis thaliana
KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 92% identity to bth:BT_3186)MetaCyc: 37% identical to glycine cleavage system L protein 1 (Arabidopsis thaliana col)
GCVMULTI-RXN [EC: 1.4.1.27]
Predicted SEED Role
"Dihydrolipoamide dehydrogenase (EC 1.8.1.4)" in subsystem Glycine cleavage system or Leucine Degradation and HMG-CoA Metabolism or Photorespiration (oxidative C2 cycle) or TCA Cycle (EC 1.8.1.4)
MetaCyc Pathways
- folate transformations II (plants) (10/11 steps found)
- folate transformations III (E. coli) (8/9 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- glycine biosynthesis II (3/3 steps found)
- glycine cleavage (3/3 steps found)
- glycine degradation (3/3 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (7/9 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (2/3 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (2/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (2/3 steps found)
- folate transformations I (9/13 steps found)
- photorespiration II (6/10 steps found)
- photorespiration I (5/9 steps found)
- photorespiration III (5/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycine, serine and threonine metabolism
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.4
Use Curated BLAST to search for 1.4.1.27 or 1.8.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (447 amino acids)
>BACOVA_02890 dihydrolipoyl dehydrogenase (Bacteroides ovatus ATCC 8483) MKYQVIIIGGGPAGYTAAEAAGKGGLSVLLIEKNSLGGVCLNEGCIPTKTLLYSAKTYDS AKHASKYAVNIPEVSFDLPKIIARKSKVVRKLVLGVKAKLTANNVTIVSGEAQIIDKNTV RCGEEIYEGENLILCTGSETFIPPIPGVDAVNYWTHRDALDSKELPASLAIVGGGVIGME FASFFNSLGVQVAVVEMMDEILGGMDKELSALLRAEYAKRGIKFLLGTKVVDLSQTGEGA VVSYENAEGNGSVIAEKLLMSVGRRPVTKGFGLENLNLEQTERGAIRVNEKMQTSVPDVY VCGDLTGFSLLAHTAVREAEVAVHSILGKEDAMSYRAIPGVVYTNPEIAGVGETEESAST KGINYQVIKLPMAYSGRFVAENEGVNGVCKVLLDEQQRVIGAHVLGNPASEIITLAGTAI ELGLTAAQWKKIVFPHPTVGEIFREVL