Protein Info for BACOVA_02614 in Bacteroides ovatus ATCC 8483

Annotation: dihydroorotate dehydrogenase 1B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 TIGR01037: dihydroorotate dehydrogenase family protein" amino acids 4 to 299 (296 residues), 376.3 bits, see alignment E=4.7e-117 PF01180: DHO_dh" amino acids 4 to 286 (283 residues), 270.9 bits, see alignment E=1.9e-84 PF01207: Dus" amino acids 93 to 278 (186 residues), 36.4 bits, see alignment E=5.2e-13 PF03060: NMO" amino acids 212 to 267 (56 residues), 32.9 bits, see alignment E=7.1e-12

Best Hits

Swiss-Prot: 98% identical to PYRDB_BACTN: Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit (pyrD) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 98% identity to bth:BT_0892)

MetaCyc: 50% identical to dihydroorotate dehydrogenase (catalytic subunit) (Bacillus subtilis subtilis 168)
Orotate reductase (NADH). [EC: 1.3.1.14]

Predicted SEED Role

"Dihydroorotate dehydrogenase, catalytic subunit (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.1, 1.3.98.1

Use Curated BLAST to search for 1.3.1.14 or 1.3.3.1 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (303 amino acids)

>BACOVA_02614 dihydroorotate dehydrogenase 1B (Bacteroides ovatus ATCC 8483)
MADLSVNIGELQMKNPVMTASGTFGYGEEFSDFIDIARIGGIIVKGTTLHKREGNPYPRM
AETPSGMLNAVGLQNKGVDYFVEHIYPRIKDIQTNMIVNVSGSAIEDYVKTAEIINELDK
IPAIELNISCPNVKQGGMAFGVSAKGASEVVKAVRSAYKKTLIVKLSPNVTDITEIARAA
EESGADSVSLINTLLGMAIDAERKRPILSTITGGMSGAAVKPIALRMVWQVAKAVNIPVI
GLGGIMDWKDAVEFMLAGATAIQIGTANFIDPAVTIKVEDGINNYLERHGCKSVKEIIGA
LEV