Protein Info for B158DRAFT_2474 in Kangiella aquimarina DSM 16071

Annotation: alkaline phosphatase (EC 3.1.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00245: Alk_phosphatase" amino acids 55 to 517 (463 residues), 429.3 bits, see alignment E=1.7e-132 PF01676: Metalloenzyme" amino acids 303 to 383 (81 residues), 26.2 bits, see alignment E=5.5e-10

Best Hits

KEGG orthology group: K01077, alkaline phosphatase [EC: 3.1.3.1] (inferred from 60% identity to cps:CPS_3932)

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (523 amino acids)

>B158DRAFT_2474 alkaline phosphatase (EC 3.1.3.1) (Kangiella aquimarina DSM 16071)
MKNFSKKCGLAVLSTLVVSSLHAAVVPDTQKNSSWFNEGQAAIQKQLALTPKNRAKNVIL
FVGDGLGVSTITAGRIFAGQKLGISGEEHQLSFDRMPYSAQVKTYNTNQQTPDSAGTMTA
MMAGVKTKAGVINIAPDAERGNCESSLGYELTTALELAEQKGLSTGVVSTARITHATPAA
TYAKVPERNWESDDNLPEEAVAAGCKDIASQLIELEAGDGIDLAFGGGRRHFLPEDTVDS
EGKNGKRKDGRDLTAEWLARYQGEAASYIETQTEFDALDIAATSKVLGLFNSSHMEYEAD
RQNDIGGEPSLSEMTGKAIEFLNKNDNGYFLMVESGRIDHAHHAGNAYRALRDTEEFAKA
VQAAVDSTNPDETLILVTADHSHVFTIAGYPQRGNPILGVVKSTDANGAPLDEPSLAADG
MPYTTVGYANGLGFADYGPGNGGDQRYGDAGLNGRVDLTTVDTTQSGFHQEALVPLGSET
HAGEDVTVHAQGPGAQWLQGVIEQNVIFHVINQAAELGGEKYQ