Protein Info for B158DRAFT_2473 in Kangiella aquimarina DSM 16071

Annotation: alpha-L-glutamate ligases, RimK family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 PF18030: Rimk_N" amino acids 1 to 97 (97 residues), 130.2 bits, see alignment E=7.2e-42 TIGR00768: alpha-L-glutamate ligase, RimK family" amino acids 2 to 289 (288 residues), 284.5 bits, see alignment E=4.2e-89 PF08443: RimK" amino acids 101 to 289 (189 residues), 226 bits, see alignment E=7.7e-71 PF02655: ATP-grasp_3" amino acids 102 to 266 (165 residues), 41.2 bits, see alignment E=4.8e-14 PF02955: GSH-S_ATP" amino acids 119 to 276 (158 residues), 58.5 bits, see alignment E=1.5e-19

Best Hits

Swiss-Prot: 79% identical to RIMK2_HAHCH: Probable alpha-L-glutamate ligase 2 (rimK2) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K05844, ribosomal protein S6 modification protein (inferred from 98% identity to kko:Kkor_1021)

MetaCyc: 60% identical to ribosomal protein S6 modification protein (Escherichia coli K-12 substr. MG1655)
6.3.2.-

Predicted SEED Role

"Ribosomal protein S6 glutaminyl transferase" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (304 amino acids)

>B158DRAFT_2473 alpha-L-glutamate ligases, RimK family (Kangiella aquimarina DSM 16071)
MKIGILSRNPRLYSTKRLVQAAEERGHEAQVVDVLKCFMNITSNRPTVHSKVQGKVRQLE
FDAVIPRIGASVTAYGTAVLRQFEVGGVYSVNESIAITRSRDKLRAHQLLARKGVGMPIT
SYAHSVEATDELIESVGGAPLIVKVIESTHGNGVVLAETNKAAESLINAFRSLNADFLVQ
EFIKEAGGSDIRCFVVGDKVIAAMQRSAKEGEFRSNLHRGGSAQVIKLKPEERALAVRAA
KVMGLDVAGVDLIRSAHGPLVIEVNSSPGLEGIEKATGKDVAGSIIQFIEKDSLKGPNKP
KGRG