Protein Info for B158DRAFT_2411 in Kangiella aquimarina DSM 16071

Annotation: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00216: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • amino acids 2 to 283 (282 residues), 334.1 bits, E=3.1e-104
• PF02401: LYTB
  • amino acids 3 to 283 (281 residues), 338.6 bits, E=1.2e-105

Best Hits

Swiss-Prot: 69% identical to ISPH_METCA: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K03527, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [EC: 1.17.1.2] (inferred from 93% identity to kko:Kkor_1661)

MetaCyc: 64% identical to 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Escherichia coli K-12 substr. MG1655)
RXN-24043 [EC: 1.17.7.4]; 1.17.7.4 [EC: 1.17.7.4]

Predicted SEED Role

"4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis (EC 1.17.1.2)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• methylerythritol phosphate pathway I (8/9 steps found)
• methylerythritol phosphate pathway II (8/9 steps found)
• isoprene biosynthesis I (8/10 steps found)
• superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.1.2 or 1.17.7.4

Protein Sequence (311 amino acids)

>B158DRAFT_2411 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2) (Kangiella aquimarina DSM 16071)
MDIVLANPRGFCAGVDRAIEIVNRAIEIFGAPIYVKHEVVHNKFVVDGLREKGAIFIEDL
NEVPEHSTLIFSAHGVSQQVRHEAKERHLKVFDATCPLVTKVHMEVSRHSRRNEECVLIG
HKGHPEVEGTMGQYDNSEAGIYLVEDEQDVAELQVRNPDSLFYVTQTTLSVDDTQRLVNA
LKDKFPNIQGPKKDDICYATQNRQDAVKQLAAVCDLILVVGAQNSSNSSRLQELAENQGC
RSYLINNATEIDASWLEGVNKVGVTAGASAPEILVQDVIKYLESQGGSTALESDGVEENI
VFVLPAELRAE