Protein Info for B158DRAFT_2408 in Kangiella aquimarina DSM 16071

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 843 (829 residues), 934.4 bits, see alignment E=3.5e-285 PF00133: tRNA-synt_1" amino acids 28 to 642 (615 residues), 477.7 bits, see alignment E=8.5e-147 PF09334: tRNA-synt_1g" amino acids 58 to 116 (59 residues), 35.5 bits, see alignment 1.5e-12 PF13603: tRNA-synt_1_2" amino acids 237 to 361 (125 residues), 26.5 bits, see alignment E=1.3e-09 PF01406: tRNA-synt_1e" amino acids 546 to 650 (105 residues), 31.3 bits, see alignment E=4.2e-11 PF08264: Anticodon_1" amino acids 687 to 842 (156 residues), 88.2 bits, see alignment E=1.6e-28 PF06827: zf-FPG_IleRS" amino acids 895 to 922 (28 residues), 37.9 bits, see alignment (E = 3.8e-13)

Best Hits

Swiss-Prot: 62% identical to SYI_ALISL: Isoleucine--tRNA ligase (ileS) from Aliivibrio salmonicida (strain LFI1238)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 62% identity to vsa:VSAL_I0580)

MetaCyc: 61% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>B158DRAFT_2408 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Kangiella aquimarina DSM 16071)
MSDYKDTLNLPKTRFEMRGNLPQREPKMLAEWSKNDLYGEIRKARAGRDQFILHDGPPYA
NGNIHIGHSVNKILKDIVIKSKTLSGFDSPYVPGWDCHGLPIENNVEKKVGKAGDKVSHA
EFRQKCRDYAAKQVDGQKTDFIRLGVLGEWDNPYLTMDFKFEANIVRALSKLVRNGHLFK
GEKPVYWSVVGGSSLAEAEVEYQDKTSFSIYVNYPVADEQAFLKAFDFAGEQGEGEVSVV
IWTTTPWTLPSSQAVTVHEDLEYVLVQVERDGKKQRFVLARELVESVMKEAEIEVYSVIA
STKGSKLENFELNHPFYDKTLPVILGEHVTTDAGTGLVHTAPDHGPDDFVVGKKYGIGLL
NYVMANGVFSDETPVFAGQHVYKVDQPVVDHLIEKGRLLHQDKMRHSYPHCWRTKTPLIY
RATPQWFISMSQNGLQAQAMEEIKKVKWVPEWGEARIESMVGSRPDWCISRQRTWGVPLC
LFIDKQTGEPHPDSADLMEKVSYYIEEKGIQAWYDLDASEFVDADKYEKIDDTLDVWFDS
GVTHYCVLEARDYLRQPADLYLEGSDQHRGWFQSSLLTATGMYGHAPYRHCLTHGFVVDG
KGEKMSKSKGNVMAPNDVINKLGADVLRLWVSSADYRGEMAVSEEILKRTGDTYRRLRNT
SRFLLSNLEGFEPERHSVAKDDLLPLDRWAVDCAYQAQQEIIKAYDDYNFWLVAQKIHHF
CSIDMGSFYLDIIKDRQYTAKTGSRAQRSCQTAMYHIIEALVRWMAPVLSFTAYEIWPLI
PGKRESNVFVAEWYEGLFPMENADITQADWALISEVKDEVNRALEAKRKDGAIGGSLAAE
VTLYANDELYAVLSKLEDELRFVFITSQARLEKGEGGEATEMKGLQLSITKSDAEKCERC
WHHRHDVGQNSEHPTICTRCVENVVGTGETRHYA