Protein Info for B158DRAFT_2407 in Kangiella aquimarina DSM 16071

Annotation: riboflavin kinase/FMN adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 16 to 169 (154 residues), 179 bits, E=6e-57
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 22 to 309 (288 residues), 314.9 bits, E=2.7e-98
• PF01687: Flavokinase
  • amino acids 187 to 308 (122 residues), 143.3 bits, E=4.5e-46

Best Hits

Swiss-Prot: 48% identical to RIBF_ECOL6: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 86% identity to kko:Kkor_1665)

MetaCyc: 48% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (1/3 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (12/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (311 amino acids)

>B158DRAFT_2407 riboflavin kinase/FMN adenylyltransferase (Kangiella aquimarina DSM 16071)
MRLIRGLYSCPPNLFQQGSIATIGNFDGVHLGHQAIIKRLTDKAKALSVPSVVIVFEPHP
QEFFRPEAAPARLFKLTDKLLALKDLGVDYVLCLRFNQELAELSAEDFIRRVLVETLHVK
HLFIGDDFKFGYKRKGDFALLQQEAKFPVEANQTVTQSIDGESARISSSLVRQAIVDNDF
EHAEKLLGRPYRFRGRVAHGDKQGRTIGVPTANIALKRVKSPLQGVYAVKVFGIKDKAIN
GVANVGIKPTLNARKERLEVHLLDFDGDVYGKQLTIEPVAKIRDEQKFSGKDELIEQINR
DIEKARLILTD