Protein Info for B158DRAFT_2361 in Kangiella aquimarina DSM 16071

Annotation: sulfate adenylyltransferase, large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 23 to 210 (188 residues), 155.2 bits, E=1.5e-49
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 25 to 430 (406 residues), 645.5 bits, E=3.7e-198
• TIGR00231: small GTP-binding protein domain
  • amino acids 31 to 209 (179 residues), 49.1 bits, E=5.6e-17
• PF01926: MMR_HSR1
  • amino acids 84 to 166 (83 residues), 24.1 bits, E=3.3e-09

Best Hits

Swiss-Prot: 69% identical to CYSN_FRATN: Sulfate adenylyltransferase subunit 1 (cysN) from Francisella tularensis subsp. novicida (strain U112)

KEGG orthology group: K00956, sulfate adenylyltransferase subunit 1 [EC: 2.7.7.4] (inferred from 96% identity to kko:Kkor_0934)

MetaCyc: 60% identical to sulfate adenylyltransferase subunit 1 (Escherichia coli K-12 substr. MG1655)
Sulfate adenylyltransferase. [EC: 2.7.7.4]

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (4/12 steps found)
• superpathway of seleno-compound metabolism (7/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Protein Sequence (473 amino acids)

>B158DRAFT_2361 sulfate adenylyltransferase, large subunit (Kangiella aquimarina DSM 16071)
MSHASPLIQEDILSYLDQHENKELLRFITCGSVDDGKSTLIGRLLHDSKMIFEDQLAAIT
EDSKKSGTTGDKVDLALLVDGLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRN
MATGASTSDLAIILIDARYGVQTQTRRHSFICSLLGIKHVIVAVNKMDLVDYSQDVYKSI
QADYLKLAGTLDIPDIRFVPISALDGDNVVNPSENMKWFRGSPLMHYLEITEIAHDINLE
DFRFPVQYVNRPNLDFRGYAGTIASGSIKVGDTIRALPSGKLSTVKSIVTFEGELEEASA
PMAVTITLNDEIDISRGDMIVKKDNLPHVSSRMRAKVVWMHEQPMTEHKEYYIKFNSKLT
TGEFDKIHHRIDVNTMVEHPAGQLELNEIGLVDLEVGEKAMFDDYRSNHKTGAFIIIDRL
SNVTVGAGMIEEPLSDSGEDSEPVEVSAYELELNQFVRKHFPHWGAKDISKSK