Protein Info for B158DRAFT_2317 in Kangiella aquimarina DSM 16071

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1012 PF13185: GAF_2" amino acids 25 to 159 (135 residues), 49.1 bits, see alignment E=3.7e-16 PF01590: GAF" amino acids 26 to 159 (134 residues), 47.4 bits, see alignment E=1.4e-15 TIGR00229: PAS domain S-box protein" amino acids 186 to 306 (121 residues), 67.4 bits, see alignment E=1.3e-22 amino acids 311 to 438 (128 residues), 44.4 bits, see alignment E=1.7e-15 amino acids 441 to 564 (124 residues), 64.1 bits, see alignment E=1.4e-21 PF00989: PAS" amino acids 191 to 297 (107 residues), 22.9 bits, see alignment E=3.6e-08 amino acids 444 to 555 (112 residues), 39.3 bits, see alignment E=3e-13 PF13426: PAS_9" amino acids 201 to 298 (98 residues), 35.7 bits, see alignment E=4.3e-12 amino acids 454 to 556 (103 residues), 30.3 bits, see alignment E=2.1e-10 PF08447: PAS_3" amino acids 339 to 423 (85 residues), 44.2 bits, see alignment E=9.5e-15 amino acids 467 to 535 (69 residues), 42.1 bits, see alignment 4.2e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 568 to 721 (154 residues), 93.5 bits, see alignment E=1.1e-30 PF00990: GGDEF" amino acids 570 to 720 (151 residues), 121.5 bits, see alignment E=1.5e-38 PF00563: EAL" amino acids 744 to 983 (240 residues), 231.3 bits, see alignment E=5.6e-72

Best Hits

KEGG orthology group: None (inferred from 88% identity to kko:Kkor_0888)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1012 amino acids)

>B158DRAFT_2317 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Kangiella aquimarina DSM 16071)
MEFESIKTNHLISIQQLATGIAKANDLQAMLDCIVDHIYSQLKSFDCALFIYESSHQELI
CYSYRKQGVSEKESFKLKPVKLGAGIIGHTAQTQRTTKVDDTDQSSIFVQDLQKNYSELC
VPILLADELIGVIDLEHPDKSFFSEEDAQYVETIASMVSYLIKQHKTEEKLHLTIEKLKF
DEKKLRDKEVFYRNLMEQASDSIFIHDQSGRFLDVNQQAVLSLGYSREKLLQMRVKDIDI
ESGQGINSGTEFYKRLRPGEPVVVECKHQRKDGSVFPVEVKVGLLTDDNIIAVARDVSKR
QQVLNELDTSKRFMQDIINTSPDVIYVYDFERDLIIDGADRFARYLGYEEDRFESWSSIL
EIIHPDDSHTLETRSDKIMNSNSDEVIKSEGRFKNANGEWVWLKNHCKIVKRTEQGLPLI
EVGTISDITHRKAMERELINKEEYYRALVENAFDGIVLYDEKGKVQFLSNSVEKLLGYKS
EDVKHKTGIDFIFPSDVELVRKAWHWVIAHPNQIYRIPDYRLVKKDGDVIWVENTLINLL
GDPVVNGIISNFRDISHKKSAEQSIYKISNYDSLTELPNRNFLRQQIERQIKKSKNGPLS
ISLVYFDIVQLNSINNAMGHWVGDQVLHYVARIIRENLDEFDLIARSGNDEFAVVLTEQN
PFEISQLVERILDSFDNIIRIGDLDLKVSLRAGIARYPEDADNAEDLLSRAEVAVGRVKN
LSTQYTFFQPQDSESIRYRINLERELVQAIDDEALELFYQPKVCLRTGVIESVEALLRWN
HPQNGYVSPQTIVNIAEANSLIYKLTSWVVENAVKQIKDWAEKGLNTRIAVNLSARDIQR
EGFEADIRSSLEKYQVDSTLLDIEITESAAMANMEHSVHTLRQLSDLGIGISLDDFGTGY
SSISYLASLPVDLVKIDQTFIQGLKQASEQAVKDNQLIIKNIIRLAESFKLVSLVEGIET
IEQCRLLQKYNCDLGQGYLFSKPKSAKDIEPLLRKGYIDMDIFKIAKPSVND