Protein Info for B158DRAFT_2277 in Kangiella aquimarina DSM 16071

Annotation: (p)ppGpp synthetase, RelA/SpoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 PF13328: HD_4" amino acids 59 to 195 (137 residues), 128.8 bits, see alignment E=3.2e-41 TIGR00691: RelA/SpoT family protein" amino acids 65 to 732 (668 residues), 622.8 bits, see alignment E=3.9e-191 PF04607: RelA_SpoT" amino acids 255 to 364 (110 residues), 149.9 bits, see alignment E=6.2e-48 PF02824: TGS" amino acids 410 to 469 (60 residues), 78.9 bits, see alignment 4.8e-26 PF13291: ACT_4" amino acids 658 to 733 (76 residues), 61.9 bits, see alignment E=1.5e-20

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 94% identity to kko:Kkor_0845)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5, 3.1.7.2

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (736 amino acids)

>B158DRAFT_2277 (p)ppGpp synthetase, RelA/SpoT family (Kangiella aquimarina DSM 16071)
MKRKDHDIFELVENSQFSFEQWLELNQLTVEGKPKSKLEKAVTMAQQNVSVTPVLKLNTL
AAGLELAEVLTHFNADFDTLTAAVLLPSVIAGSVKVETIRDKVGSTIAGLVEGAGQMEAM
RSLQQQTGAENDHQQVESLRKMLLGMVNDARVVLLKLADRVVSLRHIKDASRDIQLTFAR
ETKNIFAPLANRLGIGQLKWELEDLAFRYLEPEAYLSIAKSLKEKRVDRERYIEDVLQLL
KKKLKEENIKGEVSGRVKHIYSIWKKMTRKKVGFEEIYDVRAVRILVERVQDCYGALGIV
HGEWQHIPKEFDDYVATPKENGYRSIHTAVVGPEGKILEIQIRTFQMHEEAEKGIAAHWA
YKEGANLAKVGVDEKIAWMRQLLEWQSELADVNADELMQEFQSSVSEDRVFVFTPQGKVI
DLPAGSTPIDFAYRIHSSIGHRCVGAKINGRIVPLTYQVHTGEVIEIMTQKEESPSRDWL
IPHNGYISSSRTRHKIQQFFNKLDQEDNANAGRQLLEKELSRNDLANVPHQELAQKLHLA
SDVEMYTKIGTGNLGITQAINRAKELLESKKPQRDIQPTLRKKSKRKSYNDVSVSGVGDL
LTSIAKCCKPVPGEEIIGYITQGRGIVIHHKDCTHAKKALRDKPERIISVQWEAESTNAY
AVDLVITALDRKSLLKDITTVLANENAGVTDLSTSKRGEQVQINIEVELSQLEDLQRVIT
LLKQLPNIFKVERRRG