Protein Info for B158DRAFT_2272 in Kangiella aquimarina DSM 16071

Annotation: Signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 947 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 182 to 203 (22 residues), see Phobius details PF09984: sCache_4" amino acids 37 to 184 (148 residues), 51.1 bits, see alignment E=4e-17 PF00672: HAMP" amino acids 206 to 256 (51 residues), 35 bits, see alignment 4.4e-12 PF00512: HisKA" amino acids 301 to 365 (65 residues), 77 bits, see alignment 2.8e-25 PF02518: HATPase_c" amino acids 412 to 526 (115 residues), 97.7 bits, see alignment E=1.8e-31 PF00072: Response_reg" amino acids 694 to 804 (111 residues), 104.2 bits, see alignment E=1.3e-33 PF01627: Hpt" amino acids 852 to 932 (81 residues), 43.2 bits, see alignment E=1.1e-14

Best Hits

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 93% identity to kko:Kkor_0839)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (947 amino acids)

>B158DRAFT_2272 Signal transduction histidine kinase (Kangiella aquimarina DSM 16071)
MKDWGISKRIMTLALTPTILVALLLGTLFISNHINDSKYSLTARGKTIATHLALASEYGL
ITLDEKNLAEMAQAARDNDKDLMAVAIFDQNNRVLASVGSAETLSVMSIDNPYSLARSGS
SNNLMRFARTAESEQGKLFYAPIISKFPETYESWQNYSQANAQLLGYVAVMMTDEFSAIS
RYNTILTTLFITLIGLLIGGFLARRMSMSVTTPIEKMVEGINRIKDGRLDTRIESEANAE
LLTLQEGINLMAENMEHNQDEMQLAVDQATEDLRETLETLEVNNLELDIARRQALEASRV
KTEFLANMSHEIRTPMNGVIGFTELLLKTELNNKQKDFLFDIKRSATSLLSIIDDILDYS
KIEAGKMSFERYPFDLRECVDDIFRMLGPNANKKGIELVSLIYSDVPKALLGDPIRIKQI
ITNLVNNAIKFTKSGSVELYAEVESENKKGLKLKIQVKDSGIGLTQEQQRNLFKAFSQAD
SSTKREFGGTGLGLVISKSLVEKMGGNIGVNSNQGEGSTFWFTLQCERTDALPEDGLTGE
FLANKSVLVFDPHPSTRLSITQILEEWGMLVRSFEKIDDLMTEIDLYSQSGKSIELIIIG
GQRFRRRQQQLEYICNKAQTQLYCPIITFTTAGNADFLEHLHSIGVNRAMTKPVTHRALY
NSLIELLKTPSIHSGKEPAEAPVGDASLLKDIYVLAVDDNPANLRLVTTLLQDLNIKVDA
AESGMQAVSLSKNKVYDAILMDIQMPEMDGLEATRTIRANRTNLNTPIIALTAHAMASER
EQLLEAGMDDYMTKPVSEQALVNLLLKWTQAASDINPTPTVQQEIEDENRNQSLDWSLSL
KLANNNEQLAIDMLKMLVDSNFDTGRNIHAAYQSQDFDQLLQHVHKLHGASCYVGTPKLK
HLSNVYETLLKKQQYNKLEELHEQLLAELEVVNKEAERFLKPEKADS