Protein Info for B158DRAFT_2229 in Kangiella aquimarina DSM 16071

Annotation: 23S rRNA m(2)G-2445 methyltransferase (EC 2.1.1.52)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 718 PF02926: THUMP" amino acids 35 to 153 (119 residues), 52.3 bits, see alignment E=1.7e-17 PF01170: UPF0020" amino acids 162 to 377 (216 residues), 145.2 bits, see alignment E=5.2e-46 PF10672: Methyltrans_SAM" amino acids 493 to 668 (176 residues), 87.2 bits, see alignment E=3e-28 PF03602: Cons_hypoth95" amino acids 548 to 678 (131 residues), 37.6 bits, see alignment E=4.6e-13 PF05175: MTS" amino acids 552 to 696 (145 residues), 23.5 bits, see alignment E=9.4e-09

Best Hits

Swiss-Prot: 44% identical to RLMKL_SHESH: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Shewanella sediminis (strain HAW-EB3)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 92% identity to kko:Kkor_0795)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (718 amino acids)

>B158DRAFT_2229 23S rRNA m(2)G-2445 methyltransferase (EC 2.1.1.52) (Kangiella aquimarina DSM 16071)
MQFYASCPKGVEALLIEELTSLGATNLKEHPSHIVFEGDLELAYKACLYSRLANRIYLCL
LDEEINSVEDMYQKVNSIEWSWHFDMQQTFVVDAIARKNSDIRHSGFAAQKVKDALVDYY
KDRYEQRPSVDKDNPDYRIHAIVNGRQLKVGIDLAGRSLHQRGYRPTSGRAPIKENLAAA
LLIRAGWPELSKQGYSFHDPMCGTGTLLVEAVMIACDMAPGLFNPSFSFENWKQHDAELW
QTTVATARRQFNKNKLAINNRFIGSDSHRKSLELAKEVIRNADFGDLIELKEQDIRRPKL
EVFNEPGLLVSNPPYAERLGEEDEVMQLYRDFGEYIKSHCIGWQAAIITNEPVFAKAMGM
RSHKQYKFKNGAIDCQLYLFDVKDENFYKPFDPTVVNPDWDKNLSDGAVMLKNRIIKNQQ
RLKSYLKQNDVTCYRLYDADLPEYSAVIDVYDDMVHIQEYAPPKDIPAKVAQRRLNEIVR
VTSGLLQIPEAEIALKQRQRQKGDWQYQKQSEEEQFQVVNEQGLQFYVNLNRYLDTGLFL
DHRKTRKMIMAEAKEKRFLNLFAYTGSVSVYAAKGGAKTTTVDMSKTYLNWAIKNFGLNN
IPIYGHEFIQSDCLQWLDEAQGQRTYDLIFLDPPTFSNSKRMDTTFDVQRDQVDLIEKTM
NLLDSKGKLYFSNNFKKFEMEKELLEMFEVQEITARTLPIDFQKSRNHHRCWLIQHKS