Protein Info for B158DRAFT_2227 in Kangiella aquimarina DSM 16071

Annotation: DNA topoisomerase I, bacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876 PF01751: Toprim" amino acids 5 to 154 (150 residues), 78.6 bits, see alignment E=8.1e-26 TIGR01051: DNA topoisomerase I" amino acids 5 to 646 (642 residues), 811.7 bits, see alignment E=3.2e-248 PF01131: Topoisom_bac" amino acids 169 to 575 (407 residues), 442.1 bits, see alignment E=5.8e-136 PF01396: zf-C4_Topoisom" amino acids 609 to 647 (39 residues), 23.9 bits, see alignment (E = 7.5e-09) amino acids 721 to 756 (36 residues), 36.4 bits, see alignment (E = 8.8e-13) PF21372: TOP1_ZnF" amino acids 663 to 706 (44 residues), 73.1 bits, see alignment 3e-24 PF08272: Topo_Zn_Ribbon" amino acids 769 to 804 (36 residues), 47.9 bits, see alignment (E = 2.1e-16) amino acids 835 to 873 (39 residues), 50 bits, see alignment (E = 4.9e-17)

Best Hits

Swiss-Prot: 68% identical to TOP1_PSEAE: DNA topoisomerase 1 (topA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 69% identity to cja:CJA_2010)

MetaCyc: 65% identical to DNA topoisomerase I (Escherichia coli K-12 substr. MG1655)
5.99.1.2-RXN [EC: 5.6.2.1]

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.6.2.1 or 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (876 amino acids)

>B158DRAFT_2227 DNA topoisomerase I, bacterial (Kangiella aquimarina DSM 16071)
MGKSLVIVESPAKAKTINKYLGKDFIVKSSIGHIRDLPTGSQNKKPVDAKERARKAAATR
KLSPKEKAKQKALKEKTALFARMGINPEKSWEAQYEVLPGKEKVLNELKKLAKDADTIYL
ATDLDREGEAIAWHLRELLDGDESRFKRVVFNEITKKAIQQAFSKPGELDMNYVNAQQAR
RFLDRVVGFMVSPLLWRKISRGLSAGRVQSVAVRLLVEREREIRAFIPEEYWDIHANTLT
QSKDDLRLQVNKYQGKNFRPNSEEETNKALEVLKSSDYKVVTREDKPQKSKAPKPFITST
LQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAVESCRDYIKTNIGGEYL
PKEANSYSSKEGAQEAHEAIRPSDVAKKVGHLNGMEKDAERLYDLIWRQFVACQMLPAEF
DVTSLKVQAGDYELKARGRVMRFDGWTKVLPMQKKNAEDEELPNVKEGEMLELKKLEPKQ
HFTKPPARYSEAALVKELEKRGIGRPSTYASIISTIQDRGYVRLENRRFYAQKIGEIVTE
RLVESFNDLLDYSFTATMEKHLDDIASGRQNWKVTLDEFYDEFSKQLKKADEEEADGGMR
RNEAVETDILCEKCKTNHMAIRNASTGVFLGCMGYSLPPKERCKNTVNLIPGEEFIAADE
EEEARYLHDKKRCKKCNSTMDDYLVDENTKLYICGNNPDCDGYEIEKGEFKIKGYDGPVL
ECDKCGSDMQLKTGRFGKYFDCTNEDCKNTRKLLKNGQPAPPKADPIPMPELQCEKVDDH
YVLRDGAAGIFLAASKFPRNRETRAPAVDELITVKDKLDPKYKFLADAPLKDPDGNRTFV
KFSRKTGQQYVAGVKDDGKASGWTAFYENGKWVPQK