Protein Info for B158DRAFT_2173 in Kangiella aquimarina DSM 16071

Annotation: lysyl-tRNA synthetase, class II (EC 6.1.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR00499: lysine--tRNA ligase" amino acids 11 to 498 (488 residues), 706.5 bits, see alignment E=8.5e-217 PF01336: tRNA_anti-codon" amino acids 64 to 141 (78 residues), 68.4 bits, see alignment E=6.4e-23 PF00152: tRNA-synt_2" amino acids 157 to 496 (340 residues), 329.2 bits, see alignment E=4e-102

Best Hits

Swiss-Prot: 73% identical to SYK_IDILO: Lysine--tRNA ligase (lysS) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 95% identity to kko:Kkor_0739)

MetaCyc: 64% identical to lysyl-tRNA synthetase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]; 2.7.7.- [EC: 6.1.1.6]; 2.7.7.- [EC: 6.1.1.6]; RXN-23921 [EC: 6.1.1.6]; RXN-23924 [EC: 6.1.1.6]; RXN-23927 [EC: 6.1.1.6]; RXN0-7397 [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (499 amino acids)

>B158DRAFT_2173 lysyl-tRNA synthetase, class II (EC 6.1.1.6) (Kangiella aquimarina DSM 16071)
MSNQETPVVDVNEQITLRKQKLADKRKQGIAFPNDFRRDSLAEEIAHEYGDKEKAELEEL
GKKVKVAGRIMLFRNMGKASFITIQDMSGRIQSYVRKDAVGDEVYEDFKTWDIGDIVGIE
GTVFKTNKGELSVKASSIRLLTKSLRPLPDKWSGLSDQETRYRQRYVDLIVNQQSRETFI
VRSKVVKAIRDFLESRDFLEVETPMMHVIPGGASARPFETHHNALDMPLYLRIAPELYLK
RLVVGGLEKVFEINRNFRNEGLSTRHNPEFTMLEFYWAYADYNDLMDLTEAMLRYTVETV
LGKTVFESSGETYDFGKPFARMTVLEAIMKYQPEIKLEQLTDMDKAREVAKRLKLKLEDS
WGLGRVQIEIFEEVAEHKLIQPTFITAYPAEVSPLARRNDEDPFITDRFEFFAGGRELAN
GFSELNDAEDQADRFKAQVEAKDAGDDEAMHYDADYIRALEYGLPPTAGEGIGIDRLVML
LTDSPSIRDVLLFPHMRPE