Protein Info for B158DRAFT_2163 in Kangiella aquimarina DSM 16071

Annotation: uroporphyrin-III C-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 187 (184 residues), 190.1 bits, see alignment E=3.8e-60 PF13241: NAD_binding_7" amino acids 6 to 115 (110 residues), 96.9 bits, see alignment E=1.4e-31 PF14824: Sirohm_synth_M" amino acids 119 to 144 (26 residues), 36 bits, see alignment (E = 6e-13) TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 214 to 447 (234 residues), 309.6 bits, see alignment E=1.7e-96 PF00590: TP_methylase" amino acids 215 to 425 (211 residues), 183.9 bits, see alignment E=5.6e-58

Best Hits

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 76% identity to kko:Kkor_0729)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.107

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (480 amino acids)

>B158DRAFT_2163 uroporphyrin-III C-methyltransferase (Kangiella aquimarina DSM 16071)
MEYFPITVRLKEQRCIIVGGGEVAYRKLKSLLKAGAKVSLNALEFSSQVLELAHKHQLPV
HQRTFDEDLLDDAYLIIAATDNHQLNQQVSLAAQKRKVWVNVVDDLDLSTFIMPAIIDRS
PLLVAVSTGGVSPVLARKIREKIEWLLPRNLGQLLTRLKTFRGLIKKKFPDLKTKRSFSE
WYIEQAIGDPSFIRYGFEQSLSGYNLTNQAEKKGRVFLVGAGPGDAELLTVKALKVLQKA
DVVLHDALVSEQILEQVRRDASLIHVGKRAGKDCVPQKETNQLLVDYAEKGLTVVRLKGG
DPFIFGRGGEELEVLVDAGIEYEVIPGITAASGCASYAGIPLTHRDYAQTVMFVTAHCAN
SIDKLNWQSLAREKQTVAIYMGLLRNKTLVDNLIKHGRDGDTPIAIIENGTLPQQRVVRG
KLKQLTELVEGYKVVSPALIVIGEVTVLADRLGWFCDSHLLGSNKDNLQISTTQNLKKAS