Protein Info for B158DRAFT_2159 in Kangiella aquimarina DSM 16071

Annotation: 7-cyano-7-deazaguanine reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 TIGR03138: queuine synthase" amino acids 7 to 278 (272 residues), 419.7 bits, see alignment E=3.1e-130 PF14819: QueF_N" amino acids 18 to 127 (110 residues), 162.5 bits, see alignment E=4e-52 PF14489: QueF" amino acids 191 to 266 (76 residues), 86.3 bits, see alignment E=1.3e-28

Best Hits

Swiss-Prot: 58% identical to QUEF_VIBCH: NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K06879, 7-cyano-7-deazaguanine reductase [EC: 1.7.1.13] (inferred from 85% identity to kko:Kkor_0725)

MetaCyc: 55% identical to 7-cyano-7-deazaguanine reductase (Escherichia coli K-12 substr. MG1655)
PreQ(1) synthase. [EC: 1.7.1.13]

Predicted SEED Role

"NADPH dependent preQ0 reductase (EC 1.7.1.13)" (EC 1.7.1.13)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.13

Use Curated BLAST to search for 1.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (278 amino acids)

>B158DRAFT_2159 7-cyano-7-deazaguanine reductase (Kangiella aquimarina DSM 16071)
MQNHDTSHDIPLGKQVEYPSSYQPELLFPVARQLKRDEIDIQGQLPFHGKDIWNAYELSW
LNSKGKPQVAIAVFEFDCASTNIIESKSFKLYLNSLNQTRFTDWSIVEKTLTEDLSKASE
TTVSVKLYEVEAYNESVPVNEWRGILLDEQDIEVCDYQYDPDLLKVESEEEVSESLYSHL
LKSNCLITSQPDWASILIEYKGPKINHEALLKYLISFRNHNEFHEQCVERVFTDIMRYCE
PSELTVYARYTRRGGLDINPWRSNVKSKLCPNQRHARQ