Protein Info for B158DRAFT_2146 in Kangiella aquimarina DSM 16071

Annotation: dihydrolipoamide dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01134: GIDA
  • amino acids 7 to 68 (62 residues), 24.9 bits, E=4.3e-09
• PF07992: Pyr_redox_2
  • amino acids 7 to 326 (320 residues), 236.8 bits, E=1.4e-73
• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 7 to 457 (451 residues), 511.2 bits, E=1.2e-157
• PF00890: FAD_binding_2
  • amino acids 8 to 42 (35 residues), 20.4 bits, 1.1e-07
• PF12831: FAD_oxidored
  • amino acids 8 to 46 (39 residues), 34.1 bits, 8.4e-12
• PF00070: Pyr_redox
  • amino acids 175 to 252 (78 residues), 65.8 bits, E=1.7e-21
• PF02852: Pyr_redox_dim
  • amino acids 346 to 452 (107 residues), 129.3 bits, E=2.9e-41

Best Hits

Swiss-Prot: 70% identical to DLDH_ECOLI: Dihydrolipoyl dehydrogenase (lpdA) from Escherichia coli (strain K12)

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 96% identity to kko:Kkor_0714)

MetaCyc: 70% identical to lipoamide dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.97.1.-; Dihydrolipoyl dehydrogenase. [EC: 1.4.1.27, 1.8.1.4]; RXN-7716 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105]; RXN0-1132 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.8.1.4 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.4.1.27 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.2.1.105 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.2.1.104 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• folate transformations II (plants) (9/11 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• TCA cycle III (animals) (8/10 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• pyruvate fermentation to acetate II (2/3 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• L-tryptophan degradation III (eukaryotic) (10/15 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (2/4 steps found)
• photorespiration II (6/10 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• 2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA (2/5 steps found)
• L-lysine degradation XI (2/5 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• folate transformations I (7/13 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (9/17 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (16/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (14/26 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (11/23 steps found)
• superpathway of L-lysine degradation (9/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.2.1.104 or 1.2.1.105 or 1.4.1.27 or 1.8.1.4

Protein Sequence (472 amino acids)

>B158DRAFT_2146 dihydrolipoamide dehydrogenase (Kangiella aquimarina DSM 16071)
MSNLHAQVVVLGSGPGGYNAAFRAADLGMDVILIEKYATLGGVCLNVGCIPSKALLHTAK
VIDEAEEMSAHGVSFGKPKFDIDKIRGYKDGVIKQLTGGLAGMAKGRKVKVVQGYGKFTG
ANEIEVEHEGEKQKVTFDNAIIAAGSRVVQLPFLPEDDRIVDSTGALELRQVPKHMLVIG
GGIIGLEMATVYRALGAKISVVEMADQLVPAADKDVVKVLEKYVSNKYDNIMLETSVTKV
EAKKDGLYVTFEGKNAPEKPQKFDMILSAVGRRPNGDLIDADKAGVKVTDRGFIEVDKQM
RTNVPHIYAIGDIVGQPMLAHKATAESHVAAEVIAGKKHYFDPLTIPSVAYTDPELAWVG
LTEKEAKEQGIDYGVGKFPWAASGRAIGVNRKEGFTKLLFDKKNNRIIGGAIVGVNAGEL
IAEVGLAIEMGCDAEDIGLTIHAHPTLSESVTFAAEVYEGVCTDLPPQRKKK