Protein Info for B158DRAFT_2145 in Kangiella aquimarina DSM 16071
Annotation: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 85% identity to kko:Kkor_0713)Predicted SEED Role
"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
- pyruvate fermentation to acetate II (2/3 steps found)
- pyruvate fermentation to acetate V (2/3 steps found)
- pyruvate fermentation to acetate and (S)-lactate I (2/4 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (9/17 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (16/27 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (14/26 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.12
Use Curated BLAST to search for 2.3.1.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (552 amino acids)
>B158DRAFT_2145 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form (Kangiella aquimarina DSM 16071) MADITQVKVPDIGDASGVDVIEVLVNVGDTIDVEDSLIVLETDKATMEVPSSHAGVVKSI DVKVGDKVSEGDVILSIEATENTSAADNSEESNEEAAEEVTEADTQQEQQSADASEQDED DSDGAEEKEEPLIVPDIGDASDVDVIEVTVKAGDSVEKDDSLFVLETEKATMEVPSPSAG TIVSMSVKVGDKVSQGDQIGVIKTAATKSTKPKKQQQPADEPKPTQEAPKQADTPKPAPV SDYPVQAPTEGKLVHASPAVRRLAREFGADLTKVKGTGPKSRIMKEDVQSYIKFELSRPK ATASSGAVGTPDLPEIDFSKFGEVEQKPLSRIQKISSVNLHRNWTMIPHVTQHEDADITD LDAFRKSMKEEAAKEGVRLTPLAFIMKALVASLKAFPTFNASLANDGENLILKKYYNIGV AVDTPDGLVVPVIRDVDKKSVYELAKELGEVSEKARDKKLGAADMQGSCFTISSLGGIGG TSFTPIVNWPDVAILGLSRNQMKPVWNGKEFEPRMMLPMSLSYDHRVIDGAVAARFIVHL SKMLGDIRRVIL