Protein Info for B158DRAFT_2122 in Kangiella aquimarina DSM 16071

Annotation: UDP-N-acetylglucosamine-N-acetylmuramylpentapeptide N-acetylglucosamine transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 3 to 354 (352 residues), 359.3 bits, see alignment E=1e-111 PF03033: Glyco_transf_28" amino acids 5 to 137 (133 residues), 145.3 bits, see alignment E=2.7e-46 PF13439: Glyco_transf_4" amino acids 22 to 176 (155 residues), 36.6 bits, see alignment E=9.6e-13 PF04101: Glyco_tran_28_C" amino acids 185 to 350 (166 residues), 153.3 bits, see alignment E=1.3e-48

Best Hits

Swiss-Prot: 51% identical to MURG_AERS4: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Aeromonas salmonicida (strain A449)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 84% identity to kko:Kkor_0689)

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (372 amino acids)

>B158DRAFT_2122  UDP-N-acetylglucosamine-N-acetylmuramylpentapeptide N-acetylglucosamine transferase (Kangiella aquimarina DSM 16071)
MTKRILLMAGGTGGHIFPALAVGKELQQEGWRLHWLGSQGGMEEELVPKHDIPMSVLPVK
GIRNKGLVSLIKAPFQLMKSVWLARKAIKQFKPDVVLGMGGFASGPGGIAAKLCGVPLVI
HEQNAIAGMTNTQLSRFSRWTLQAYPGAFPESDKVKTVGNPVRQDIGSDKDPSERIEMDN
RSVHILVVGGSRGAAVFNEELPETFSLVNSGLEVHVKHQCGKGNYESVTERYEATATDKV
KFEVCEFIDDMAHAYQWADLVVCRAGALTVAEIAMAGCVAIFVPYPHAVDDHQTYNARYL
ADQGAALIIQQHDLGKERLAQEITALANDKEHLIEMARKSQALARTDATKAVAEYCKQAA
AEKAETKVRNKT