Protein Info for B158DRAFT_2120 in Kangiella aquimarina DSM 16071

Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21799: MurD-like_N
  • amino acids 12 to 99 (88 residues), 74.9 bits, E=7.7e-25
• TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase
  • amino acids 15 to 445 (431 residues), 434 bits, E=3.8e-134
• PF08245: Mur_ligase_M
  • amino acids 115 to 286 (172 residues), 99 bits, E=5.7e-32
• PF02875: Mur_ligase_C
  • amino acids 309 to 385 (77 residues), 42.3 bits, E=1.1e-14

Best Hits

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 78% identity to kko:Kkor_0687)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Protein Sequence (447 amino acids)

>B158DRAFT_2120 UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) (Kangiella aquimarina DSM 16071)
MMAMQHKDIAYKNRLILGLGQTGLAVARYLSALRQPFHVMDTRDIAPGHEELEKLAPGAL
LKWNGELFVNYDELVVSPGISIARDDIQAARNAGVSIVGDIELFAQINSLPVIAITGSNG
KSTVTDLVGELINASGKRALVGGNIGVPVLDLLEQDGDLVALELSSFQLETTDSLKPVVA
TVLNVSDDHLDRYNSFQDYADAKLRIYQQAGTCLINADDKNTWLKDASGKAQVVKFGIAN
EHTQPEWAVNIEQNSITHNGNKVLALNELTLQGIHNALNVAVALALVEIAGIKLTKPVLD
AAKSYKGLPHRCQLVAVKNGVTFINDSKATNVGATVAAILSFQPQFGNNIVLIAGGDAKG
ADLSSLRQIVNETVKAVVCFGKDAPAIAKLCPQKSQLVGNLKEAVTTANQYAQEGDLVLL
SPACASLDMFANYMHRGEQFAQCVEAL