Protein Info for B158DRAFT_2101 in Kangiella aquimarina DSM 16071

Annotation: Cytochrome b subunit of the bc complex

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 34 to 60 (27 residues), see Phobius details amino acids 80 to 104 (25 residues), see Phobius details amino acids 116 to 138 (23 residues), see Phobius details amino acids 145 to 162 (18 residues), see Phobius details amino acids 183 to 205 (23 residues), see Phobius details amino acids 244 to 264 (21 residues), see Phobius details amino acids 306 to 325 (20 residues), see Phobius details amino acids 342 to 362 (21 residues), see Phobius details amino acids 368 to 387 (20 residues), see Phobius details PF00033: Cytochrome_B" amino acids 25 to 210 (186 residues), 220.6 bits, see alignment E=2.4e-69 PF13631: Cytochrom_B_N_2" amino acids 92 to 262 (171 residues), 150.1 bits, see alignment E=8.9e-48 PF00032: Cytochrom_B_C" amino acids 276 to 378 (103 residues), 138.2 bits, see alignment E=1.7e-44

Best Hits

Swiss-Prot: 63% identical to CYB_ALLVD: Cytochrome b (petB) from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D)

KEGG orthology group: K00412, ubiquinol-cytochrome c reductase cytochrome b subunit [EC: 1.10.2.2] (inferred from 97% identity to kko:Kkor_0668)

MetaCyc: 50% identical to cytochrome b (Acidithiobacillus ferrooxidans)
RXN-15829 [EC: 7.1.1.8]

Predicted SEED Role

"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2 or 7.1.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>B158DRAFT_2101 Cytochrome b subunit of the bc complex (Kangiella aquimarina DSM 16071)
MFKNFMTWLDARFPATKVWNEHLAEYYAPKNFNFWYFFGSLAMLVLVNQLLTGIWLTMFY
SPSEPFASVEYIMRDVEYGWLIRYLHSTGASAFFVVVYLHMFRGIMYGSYKKPRELIWIF
GMIIFVLLMAEAFMGYLLPWGQMSFWGAQVIVNLFGTIPVVGPDLVEWIRGDFTLSLATL
NRFFALHVVAVPLALVAMVFLHIVALHKVGSNNPDGVEIKEKKDENGIPLDGIPFHPYYT
VKDIVGVVVFMMIFLGVVFFAPDFGGYFLERPNFEPANPLKTPPHIAPVWYFTPFYTMLR
AVPDPFGGFIVMALAIVILFLLPWLDRQKVKSIRYRGASYKILLALFVISFIILGVLGVV
PVTDFTKWLGRICTVYYFFFFIALWFLPKFEKTKPVPERVTDK