Protein Info for B158DRAFT_2047 in Kangiella aquimarina DSM 16071

Annotation: ATP-dependent RNA helicase DbpA (EC 5.99.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF00270: DEAD" amino acids 28 to 194 (167 residues), 174.4 bits, see alignment E=3.4e-55 PF04851: ResIII" amino acids 40 to 189 (150 residues), 39.9 bits, see alignment E=8.2e-14 PF00271: Helicase_C" amino acids 234 to 338 (105 residues), 104.9 bits, see alignment E=5.8e-34 PF03880: DbpA" amino acids 388 to 458 (71 residues), 73.3 bits, see alignment E=2.8e-24

Best Hits

Swiss-Prot: 54% identical to DBPA_ECOLI: ATP-dependent RNA helicase DbpA (dbpA) from Escherichia coli (strain K12)

KEGG orthology group: K05591, ATP-independent RNA helicase DbpA [EC: 3.6.4.13] (inferred from 89% identity to kko:Kkor_0617)

MetaCyc: 54% identical to ATP-dependent RNA helicase DbpA (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent 23S rRNA helicase DbpA"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (460 amino acids)

>B158DRAFT_2047 ATP-dependent RNA helicase DbpA (EC 5.99.1.-) (Kangiella aquimarina DSM 16071)
MSHAAFSTLALQPELIANLADLNFEQMTPIQADALPLVLEGKDVIAQAKTGSGKTAVFGL
GILEKLDTKNFSVQSLVLCPTRELAEQVAEEIRRLARPIANVKVITACGGTPLRPQADSL
KHGAHIVVGTPGRIVDHLEKETIDLSSMTTFVLDEADRMLDMGFQPALDTIIESLPPKRQ
TLLFSATYPKQIQSIAERVMTQPVLVKALSKHDNSSIEQDFYKISDEGERDVAVRLLLLK
HRPDSTVIFCNTKRHVKDLTQELKRHGFSVLALHGDLEQKERDQALVRFANNSVSIMVAT
DVAARGLDIDSLDLVINYHIASDPEVHVHRVGRTGRAGNKGHACSIVINKESHKLARLAE
YLEQEITPQPLPDTKLLKEPTFRPPMSTIQIDGGKKQKLRPGDILGALTADYRIEGSDVG
KIQVANSWAYVAVKTDVLETAVQLLNNGKMKGKKFRARKI