Protein Info for B158DRAFT_2012 in Kangiella aquimarina DSM 16071

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 PF00772: DnaB" amino acids 17 to 116 (100 residues), 112.1 bits, see alignment E=1.9e-36 TIGR00665: replicative DNA helicase" amino acids 17 to 452 (436 residues), 556.9 bits, see alignment E=1.6e-171 PF03796: DnaB_C" amino acids 193 to 450 (258 residues), 351.6 bits, see alignment E=3.7e-109 PF13481: AAA_25" amino acids 198 to 373 (176 residues), 35.8 bits, see alignment E=9.6e-13

Best Hits

Swiss-Prot: 61% identical to DNAB_ECOL6: Replicative DNA helicase (dnaB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 98% identity to kko:Kkor_0578)

MetaCyc: 61% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>B158DRAFT_2012 replicative DNA helicase (Kangiella aquimarina DSM 16071)
MLTANQSDNKIKDLKVPPHSIEAEQSVLGGVMLDNEVWEMVSEMLLSTDFYVKNHREIFK
AMEELANMAKPMDVITLSEHLEHKGESDIGLAFLGDLAQNTPSTANIRAYAEIVREKAIM
RQLISASNEIADASFNPKGRSLAELMDMAERKVFAISQAREASGEGPSKVEDILKSTIQR
IETIQKTKGGITGLATGFTDLDKMTSGLQAGDLVIVAARPSMGKTTFAMNIVENVALNQE
KPVLVFSLEMPSESIIMRSISSLGRLEQNKIRSGQIQSSEDWDKFNSGVLQLKNHTKLLI
DDAGGLSPAEMRSRSRRVAREYGSVGLIMVDYLQLMQVPGMSDNRTLEVSEISRSLKALA
KELEVPVIALSQLNRSLEQRSDRRPVMSDLRESGAIEQDADLILFIYRDEVYHPETERKG
IAEIKIGKQRNGPIGSVELSFQGQFCRFDNLAHQDYEAQGY